Page 36 - Zbornik_Konf_2019

https://doi.org/10.15414/2019.9788055220703

4 International Scientific Conference Abstracts Book
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EFFECT OF SEED INOCULATION WITH NODULE BACTERIA ON SOYBEAN YIELD

Iryna Gumeniuk, Oleksandr Botsula
Institute of Agroecology and Environmental Management of National Academy of Agrarian Sciences of
Ukraine, Kyiv, Ukraine; E-mail.: gumenyuk.ir@gmail.com
Soybean is quite demanding on the soil, its fertility, moisture supply and reaction of the
soil solution. It is known that the formation of the harvest of any cultivated crop, including
soybeans, occurs already from the initial phases of their growth and development and
depends on many environmental factors during its growth.
The aim of our work that makes it possible to evaluate the effectiveness of the
application of various agricultural methods for improving the conditions for growing crops
was their impact on the yield and quality of the final plant products. The average yield when
using biological products based on nitrogen-fixing microorganisms is
2.11 t/ha. Field experiments were carried out according to the generally accepted method of a
field experiment in crop production (Dospehov, 1985), accompanied by observations,
definitions, accounting, and analysis. The repetition of the experience of four times, the
placement of areas randomized. The sown area was 25 m², the registration area – 10 m². The
research results indicate that the highest yield was owned by the variant using the adapted
Bradyrhizobium japonicum isolate LG 5 since the yield increase was 29.2 % relative to the
control.
The yield of soybean seed varieties Moravia for the actions of the culture fluids of the
studied soybean nodule bacteria was analyzed. It is known that the use of microbial
preparations contributes to an increase in seed productivity since for such a legume culture as
soybean it is very important to have an adequate and optimal level of nitrogen supply. Only
under the condition of a highly efficient partnership of macro- and microsymbiont and as a
result of the enhancement of a number of physiological and biochemical processes, growth
processes and the formation of seed yield are activated.
As a result of the analysis of the conducted research, it was established that the yield of
soybean was formed depending on weather conditions and the use of culture liquids of nodule
bacteria. It was revealed that over the years of field studies of isolates of B. japonicum LG 2
and LG 5, depending on the type of experience, the yield was within 2.56–2.97 t/ha. The
results of the research indicate that the treatment of the culture fluid of the new isolates
contributed to the increase in yield. seeds of soybean plant Moravia and the yield increase
was 22.4–29.2 % relative to the control.
The use of the culture fluid of the isolate B. japonicum LG 5 contributed to an increase in
yield by 4.4 % compared with the variant with the use of the drug Rizoaktiv. In the variant
with the use of B. japonicum LG 2 isolate, the harvest was at a level using the presowing
bacterization of soybean seeds with Rizoaktiv preparation.
Thus, our experimental data on soybean yields are consistent with our research on the
passage of biological processes in plants and soil and indicate that the increase in productivity
of soybean plants is, on the one hand, a consequence of the functioning of the Bradyrhizobium
japonicum – Glycine max (L.) Merrill symbiotic system. Specifically, such features as a
significant nodulation ability, high nitrogenase activity, virulence and competitiveness of
nodule bacteria isolated from the soil and presented in our research, influence the course of
the main physiological processes in soybean plants, giving an opportunity to get a sustainable
and organic soybean harvest.

Keywords: soybean, yield, Bradyrhizobium japonicum, symbiotic system, nitrogenase activity.

|36 4 International Scientific Conference Agrobiodiversity Nutrition, Health and Quality of Human and Bees Life
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September 11–13, 2019