Page 32 - Zbornik_Konf_2019

https://doi.org/10.15414/2019.9788055220703

4 International Scientific Conference Abstracts Book
th
ANTIANEMIC PROPERTIES OF MEDICINAL PLANTS

Kateryna Garkava , Kateryna Dovgopola , Ulyana Tymoshenko 2
1
1
1 National Aviation University, Kyiv, Ukraine; E-mail.: immunolog@ukr.net
2 Government Agency 'Institute of Hematology and Transfusiology National Academy of Medical
Sciences of Ukraine', Kyiv, Ukraine

Anemia is a disease that is spread throughout the world and occurs as a result of
lowering erythrocytes and hemoglobin in the blood. The causes of anemia are different. This
may be a lack of trace elements or a deficiency of vitamins B6, B9, B12 (folic acid deficiency
anemia), and in the case of acute bleeding, post-hemorrhagic anemia. Hemolytic anemia
develops in the destruction of erythrocytes, and if defective hematopoiesis is hypoplastic
anemia. The listed forms of anemia are rare, which can not be said about iron deficiency
anemia. In this form of anemia in Europe, 40 % of women and 15 % of men are ill. The daily
dose of elemental iron should not exceed 100 mg and is divided into 3–4 meals before meals,
but it does not always have a positive effect, so the use of medicinal plants with anti-anemic
properties is currently a very topical problem. Why medicinal plants? The plant is a biofactory
that produces many biologically active compounds of different orientations. Plants are used as
reductive therapy, which restores the activity of enzymes, dietary therapy, which introduces
into the body insufficient products of intermediate metabolism and detoxification.
In view of this, the purpose of this work was to study the effect of water – salt extracts of
medicinal plants on the osmotic resistance of erythrocytes. The following plants were used as
experimental samples: Hypericum perforatum L.; Trifolium pratense L.; Taraxacum officinale
Wigg; Plantago major L.; Cichorium intybus L.
Osmotic resistance of erythrocytes, which was received from the blood of donors, was
determined by the level of their hemolysis. For these erythrocytes were treated with 0.1 %
water-salt extracts from plants. To obtain aqueous-salt extractors, a solution of 0.15 mol/L of
NaCl was used. The resistance of erythrocytes was determined by the percentage of hemolysis
in buffered isotonic solutions of sodium chloride of different concentration from 0.1 to 1.0 %
after the effect on them of water-salt extracts of experimental plants. The state of osmotic
resistance of erythrocytes depends on membrane permeability, the level of free radical
processes and the state of antioxidant systems. Controls were erythrocytes that were not
treated with extracts of experimental plants.
The obtained results showed that experimental plants increased the osmotherapy
resistance of erythrocytes, but differently. So Hypericum perforatum, depending on the growth
site, reduced the erythrocyte hemolysis from 10 to 20 %, Trifolium pratense –decreased by
10–15 %, Taraxacum officinale – by 30–35 %, Plantago major – by 10–20 %, Cichorium intybus
– 10–30 %. Although, according to folk medicine, only Hypericum perforatum, Trifolium
pretense, and Plantago major are influenced by hemostasis from our experimental plants.
Previous studies of Rosa cinnamomea L. and Crataegus oxycantha showed that these plants
have high anti-anemic properties.
Thus, it is necessary to expand the range of plant studies that may have potential anti-
anemic properties, taking into account their place of growth and individual sensitivity to
them.

Keywords: erythrocytes, medicinal plants, osmotic resistance.

|32 4 International Scientific Conference Agrobiodiversity Nutrition, Health and Quality of Human and Bees Life
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September 11–13, 2019