Page 39 - Zbornik_Konf_2019

https://doi.org/10.15414/2019.9788055220703

4 International Scientific Conference Abstracts Book
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CHEMICAL COMPOSITION OF SATUREJA MONTANA L. CULTIVATED IN UKRAINE

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Nataliia Hudz , Marietta Białoń , Liudmyla Svydenko , Piotr Paweł Wieczorek
1 Danylo Halytsky Lviv National Medical University, Lviv, Ukraine;
E-mail.: nataligudz03021972@gmail.com
2 University of Opole, Opole, Poland
3 Sector of mobilization and conservation of plant resources of the Rice Institute of the NAAS,
Plodove, Ukraine
Satureja montana L., commonly known as winter savory or mountain savory, belongs to
the Lamiaceae family. It is a perennial semishrub (20–30 cm) that inhabits arid, sunny, and
rocky regions. The purpose of our study was to determine volatile compounds of S. montana

aerial parts cultivated in Plodove (Ukraine).
The volatile compounds of S. montana aerial parts were identified by comparing the
mass spectra data with spectrometer database of the NIST 11 Library and by comparison of
their retention index calculated against n-alkanes (C9–C20). Each chromatographic analysis
was repeated three times. The average value of the relative composition of the essential oil
percentage was calculated from the peak areas. The Hewlett Packard HP 6890 series GC
system chromatograph was used for the study, which was coupled with the Hewlett Packard
5973 mass selective detector (Hewlett Packard, Waldbronn, Germany). The chromatograph
was equipped with the non-polar, high-temperature ZB-5HT (5 % diphenyl, 95 %
dimethylpolysiloxane) capillary column; length, 30 m; inner diameter, 0.32 mm; film
thickness, 0.25 μm (Phenomenex Inc., Torrance, CA, USA). The gas chromatograph was
equipped with a split injector; the split ratio was 20:1 and 1 μm of a sample was introduced.
Helium served as the carrier gas, and its flow rate was 2 mL/min. Analyses were performed at
a temperature of 40–280 °C and the heating rate was 10°C/min. Injector temperature was 250
°C.
According to the published data, essential oil of S. montana consists mainly of carvacrol
(0.1–53.35 %), p-cymene (0.66–41.4 %), thymol (0.15–46.0 %), linalool (0.1–50.42 %), and
other monoterpenoids, as well as sesquiterpenes and diterpenes. Based on an analysis of the
chemical composition of the S. montana essential oil, currently, some chemotypes are
identified. Among them are chemotype of thymol and carvacrol, chemotype of linalool,
p-cymene, and α-terpineol, chemotype of linalool, intermediate chemotype of
p-cymene/linalool, chemotype of p-cymene, thymol and carvacrol, chemotype of myrcene and
viridiflorol.
Based on the experimental studies conducted, the authors established the presence of a
special chemotype of S. montana grown on experimental plots in the Kherson region.
p-Thymol was dominated (about 81.79 %) in its essential oil. In general, 34 compounds were
identified. p-Thymol has not been stated earlier in the available scientific publications as the
dominant component of the S. montana essential oil. Among minor components was linalool
2.09 %, o-cymene 1.26 %, cis-β-terpineol 1.21 %, terprinen-4-ol 1.07 %, thymol 0.65 %,
carvacrol 0.05 %. According to literature data, essential oils in which such aromatic
substances as carvacrol, eugenol or thymol dominate have a pronounced antimicrobial action.
Hajdari et al. (2016) stated the absence of carvacrol in the myrcene and viridiflorol
chemotype and linalool chemotype. These chemotypes contained also minor amounts of
thymol (0.15–0.79 %).
As a result of the performed studies, the new chemotype of S. montana was identified.
Keywords: Satureja montana, essential oil, p-thymol.

4 International Scientific Conference Agrobiodiversity Nutrition, Health and Quality of Human and Bees Life |39
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September 11–13, 2019