Page 34 - Zbornik_Konf_2019

https://doi.org/10.15414/2019.9788055220703

4 International Scientific Conference Abstracts Book
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COMPARISON OF ANTIOXIDATIVE PROPERTIES OF FRUIT POMACE FROM DIFFERENT
CULTIVARS OF HONEYSUCKLE BERRY
Marta Gołba, Anna Sokół‐Łętowska, Alicja Kucharska

Department of Fruit, Vegetable and Plant Nutraceutical Technology,
Faculty of Biotechnology and Food Science, Wroclaw University of Environmental and Life Sciences,
Wrocław, Poland; E-mail.: marta.golba@upwr.edu.pl

According to the current studies, honeysuckle berry fruit show antioxidant, anti-
inflammatory, anti-cancer properties, as well as cardio and neuroprotective. Researchers have
analyzed a vast content of iridoids and phenolic compounds in the fruit. Additionally,
numerous cultivars of honeysuckle berry have been identified. Many researches depict that
existing cultivars differ in the content of anthocyanins, flavonoids, phenolic acids. The
compounds being present in the fruit are able to neutralize reactive oxygen species, which is
mostly associated with the presence of polyphenols. Therefore, it becomes crucial to
determine the cultivars with the greatest content of bioactive compounds and best health
properties. An important aspect of the research is focusing on the side product being formed
during juice pressing-pomace. They are often considered as a waste product, while they might
be a source of vital bioactive substances. An analysis of the compounds contained in
honeysuckle berry pomace could potentially correspond to minimizing the waste product and
thus decreasing the production costs.
The aim of the research was to determine the antioxidant activity of analyzed fruit
pomace its being important in terms of science and application and to compare the impact of
different extraction conditions.
In this study, the pomace of 5 honeysuckle berry cultivars were analyzed: Jugana, Honey
Bee, Indigo Gem, Wojtek Docz Velikana. The antioxidant activity was measured using ABTS,
FRAP, DPPH methods. The extracts for the analysis were prepared using 8 different
conditions: 25 % methanol + 1ml/L HCl, 50 % methanol + 1ml/L HCl, 80 % methanol + 1ml/L
HCl, 100 % methanol + 1ml/L HCl, H2O 100 C, H2O 40 C, 25 % methanol, 50 % methanol.
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Among the analyzed cultivars, the highest ABTS value showed Wojtek, while the lowest
activity indicated Docz Velikana (1.63 mM Tx/100 g). In terms of FRAP the highest result was
measured in cultivar Jugana (12.03 mM Tx/100 g), and the lowest in Docz Velikana (1.45 mM
Tx/100 g). The last analysis- DPPH, showed the highest activity of Jugana (4.65 mM Tx/100
g), and the lowest of Docz Velikana (0.31 mM Tx/100 g). In terms of extraction method, more
concentrated methanol (50 % and more) provided in more effective extraction.
It can be concluded that fruit pomace shows antioxidant activity and the differences
between various varieties can be observed. Pomace is, therefore, a promising source of
bioactive compounds and do not have to be considered as a waste product.

Keywords: honeysuckle berry, cultivars, antioxidant, fruit pomace.

|34 4 International Scientific Conference Agrobiodiversity Nutrition, Health and Quality of Human and Bees Life
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September 11–13, 2019