Page 40 - Zbornik_Konf_2019

https://doi.org/10.15414/2019.9788055220703

4 International Scientific Conference Abstracts Book
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DETERMINATION OF THE TOTAL PHENOLIC CONTENT IN THE TINCTURE OF PROPOLIS
OF UKRAINIAN ORIGIN
Nataliia Hudz , Olena Tkachenko , Ján Brindza
1
3
2
1 Danylo Halytsky Lviv National Medical University, Lviv, Ukraine;
E-mail.: nataligudz03021972@gmail.com
2 National University of Life and Environmental Sciences of Ukraine
3 Institute of Biodiversity Conservation and Biosafety, Slovak University of Agriculture in Nitra, Slovak
Republic

Propolis consists of a complex mixture of resinous substances collected by honeybees
from different plant sources. The most abundant compounds are phenolics including aromatic
alcohols, phenolic acids and their esters (caffeic acid, cinnamic acid, p-coumaric acid and,
benzoic acid), flavonoids (chrysin, dihydrochrysin 2´,6´-dihydroxy-4´-methoxy chalcon,
galangin, and pinocembrin), and fatty acids. The typical raw propolis consists of 45–55 %
plant resin, 25–35 % of wax, 5–10 % of essential and aromatic oil, 5% of pollen, 5% of other
natural products, and secondary plant metabolites, which differ in concentrations depending
on season, geographic origin of the collection and the proximity of a beehive to particular
plant sources. Several groups of researchers documented that all types of propolis have
antibacterial properties (Huang et al., 2014; AL-Ani et al., 2018).
The purpose of this work was to determine the total phenolic content in the tincture of
propolis of the Ukrainian origin.
The propolis sample was collected in Ukraine on 16 September 2017. The hives were
located in Vradivka of Mykolajiv region. Propolis was extracted with 60 % ethanol at a ratio of
propolis to the solvent 1:12 (w:v) for the appropriate time.
Tincture of propolis could be considered as a component of antimicrobial medicinal
products, toothpastes, teeth elixirs against drug-resistant microorganisms.
Propolis is rich in phenolics. The quantity of phenolics could be used as a criterion to
evaluate the quality of propolis. The total phenolic content (TPC) of the propolis tincture was
determined according to the Folin–Ciocalteu assay and expressed in g of gallic acid
equivalents per 1L of the tincture.
TPC of the tincture was determined according to the following analytical procedure.
100 µL of the tincture dilution (1:200) was mixed with 100 µL of the Folin–Ciocalteu reagent,
later 1500 µl of purified water and 300 µL of 20 % solution of sodium carbonate were added.
The mixture was mixed by vortex and incubation was done for 60 minutes at room
temperature at the darkness. Absorbance was read at 760 mm using spectrophotometer
«Genesys 20». Purified water was used as a blank. The test was carried out for the tincture in
triplicate. The mean of three readings was used for calculations of mean TPC. The results
were expressed as gallic acid equivalents: grams eq-gallic acid per 1 liter of the tincture. It was
established that the prepared tincture contained a very high TPC: 15.849 g per 1 liter.
As a result of the performed studies, the TPC was determined in the tincture of propolis
of the Ukrainian origin.

Keywords: propolis, Folin-Ciocalteau reagent, polyphenols.
Acknowledgments
These studies were supported by a scholarship from Slovak Academic Information Agency (SAIA) and
with the active participation of researchers in international network AgroBioNet.

|40 4 International Scientific Conference Agrobiodiversity Nutrition, Health and Quality of Human and Bees Life
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September 11–13, 2019