Page 35 - Zbornik_Konf_2019

https://doi.org/10.15414/2019.9788055220703

4 International Scientific Conference Abstracts Book
th
POLLEN MORPHOLOGY OF SOME SPECIES OF THE GENUS AMELANCHIER MEDIK.

Olga Grygorieva , Ivan Gurnenko , Svitlana Klymenko , Svetlana Motyleva
1
1
1
2
1 M.M. Gryshko National Botanical Garden of the National Academy of Sciences of Ukraine,
Kyiv, Ukraine; E-mail.: olgrygorieva@gmail.com
2 Federal State Budgetary Scientific Institution All-Russian Horticultural Institute for Breeding,
Agrotechnology and Nursery, Moscow, Russian Federation
Pollen grain structure is one of the diagnostic taxonomic and phylogenetic parameters.
Study of morphology and morphometry of pollen grains of Amelanchier spp. allows found new
additional diagnostic parameters of species.
The aim of this study was to compare the morphological parameters of pollen grains of
the five species of Amelanchier spp.
Pollen grains studied on the species collected in Forest-Steppe of Ukraine in M.M.
Gryshko National Botanical Garden of NAS of Ukraine (NBG). The following species were
analyzed: Amelanchier alnifolia (Nutt.) Nutt. ex M. Roem., Amelanchier arborea (F.Michx.)
Fernald, Amelanchier canadensis (L.) Medik., Amelanchier lamarckii F.G. Schroed., Amelanchier
spicata (Lam.) K. Koch. An investigation carried out at the laboratory of Department of
Tropical and Subtropical plants of NBG using an electron microscope Carl Zeiss LS 15. The
measurement of morphometric parameters was carried out on 50 pollen grains from each
species using the AxioVision Rel. 4.8.2.0 program. The measurements were made in
micrometer (μm). The length of a polar axis (P) and the equatorial diameter (E) of grain, P/E
ratio were measured and their variation was compared among studied species.
SEM investigations showed that the pollen grains species of Amelanchier are oblong-
spheroidal, 3rd furrow is meridional, in outline from the pole are three furrow - the furrows
are long, narrow, smooth. The surface sculpture and shape index of the species vary. The
average length of the polar axis varied from 13.03 to 21.37 μm and the width of the equatorial
axis was in the range from 6.55 to 11.96 μm. Shape index (P/E) of tested species varied from
1.54 to 2.33. The most average length of pollen was Amelanchier spicata (20.17 μm),
Amelanchier canadensis (19.46 μm), the least length was Amelanchier arborea (13.69 μm). The
most average width pollen у Amelanchier lamarckii (10.59 μm), Amelanchier canadensis
(10.11 μm), and the least width was Amelanchier arborea (7.15 μm).
Pollen grains of Amelanchier alnifolia among other species the longest from the poles,
slightly pointed. Ultra-sculpture of exine is finely trickled, often perforated on all surface.
Pollen grains of Amelanchier arborea long from poles, rounded. Ultra-sculpture of exine is
wide streaming and less perforated then Amelanchier alnifolia. Ultra-sculpture of Amelanchier
canadensis exine is pronounced streaky, dense, relief, perforation not often, close to poles.
Pollen grains of Amelanchier lamarckii and Amelanchier spicata had the most equatorial
diameter and more rounded shape than other species. Amelanchier lamarckii ultra-sculpture
width streaky, sometimes intermittent, perforated on all surface. Ultra-sculpture of exine of
Amelanchier spicata not perforated, intermittently finely trickle.
Studies have established characteristic differences in the morphometric and
microsculptural features of pollen for each of the studied species of Amelanchier spp.
Keywords: Amelanchier spp., pollen, SEM, morphology.
Acknowledgments
The publication was prepared with the active participation of researchers in international network
AgroBioNet, as a part of the international program 'Agricultural Biodiversity to Improve Nutrition,
Health and Quality of Life', within the project ITEBIO-ITMS 26220220115 'Support of Technologies
Innovation for Special Bio-Food Products for Human Healthy Nutrition' and National Scholarship
Programme of the Slovak Republic and Polish National Commission for UNESCO.

4 International Scientific Conference Agrobiodiversity Nutrition, Health and Quality of Human and Bees Life |35
th
September 11–13, 2019