Page 41 - Zbornik_Konf_2019

https://doi.org/10.15414/2019.9788055220703

4 International Scientific Conference Abstracts Book
th
ANTIOXIDANT ACTIVITY OF LEAF EXTRACTS OF
CRAMBE KOKTEBELICA (JUNGE) N. BUSCH AND CRAMBE MITRIDATIS JUZ.
Mariia Kalista , Nataliia Nikolaieva , Ján Brindza 2
2
1
1 National Museum of Natural History of the National Academy of Sciences of Ukraine,
Kyiv, Ukraine; E-mail.: crambe@ukr.net
2 Institute of Biological Conservation and Biosafety, Slovak University of Agriculture in Nitra,
Slovak Republic
Crambe koktebelica (Junge) N. Busch and Crambe mitridatis Juz. are one of the forgotten
and underutilized germplasms for vegetable production. The chemical and biological features
of these species show the necessity for their widespread introduction and cultivation in
farming. The aim of this study was to investigate the antioxidant activity of water and
methanolic extracts of Crambe koktebelica and Crambe mitridatis since antioxidants play an
important role in food preservation by inhibiting oxidation processes and contributing to
health promotion rendered by many dietary supplements, nutraceuticals, and functional food
ingredients. Obtained results will be useful for the purpose of using them in farming also as
medicinal and food plants.
Plant materials of Crambe koktebelica and Crambe mitridatis for studying were collected
during expeditions to the Crimea in 2010-2013. Leaves of both species were collected from
their locus classicus (in Karadag array for the first one and in the Kerch Peninsula for the
second species). The amount of 1 g of dried and powdered leaves was extracted with
methanol (25 ml) and distilled water (25 ml). The infusions were stirred on the magnetic
stirrer at room temperature for 5 h. This was then centrifuged at 6000 rpm at 4° for 10 min
and the supernatant was stored at -4° for further analysis. The antioxidant activity was
determined by a spectrophotometric method based on a percentage of inhibition of DPPH
radical (2,2-diphenyl-1-picrylhydrazyl). We determined the optical density of solutions in the
interaction DPPH with extractive substances of plants by spectrophotometer "Genesis 20" at a
wavelength of 515 nm.
The antioxidant activity of water extract of Crambe koktebelica was 50.17±0.44 %, the
highest value of investigated species. Methanolic extract of Crambe koktebelica had a value of
antioxidant activity 26.32±0.74 %. Crambe mitridatis water and methanolic extracts showed
lower antioxidant activity 22.80±6.21 and 15.39±0.17 %. Accordingly, Crambe koktebelica
plant material could be a potential natural source of antioxidants and could have greater
importance as a therapeutic agent in preventing or slowing oxidative stress-related
degenerative diseases.
The results demonstrated that both species can be used for the food industry because of
their relatively high value of the antioxidant activity, especially water extracts. They are
promising for use in fodder, food, decorative and medicinal purposes. These species have got
95 % germination of seeds, simple agrotechnology and combination of fairly high seed and
fruit weight indexes with an average of actual seed productivity. Therefore, cultivation of
Crambe koktebelica and Crambe mitridatis is sufficiently easy and doesn’t need many
investments.

Keywords: antioxidant activity, Crambe koktebelica, Crambe mitridatis.

Acknowledgments
This work was supported by the International Visegrad Scholarship Fund (51700619) at the Institute
of Biodiversity Conservation and Biosafety. The publication was prepared with the active participation
of researchers in international network AgroBioNet.

4 International Scientific Conference Agrobiodiversity Nutrition, Health and Quality of Human and Bees Life |41
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September 11–13, 2019