Page 44 - Zbornik_Konf_2019

https://doi.org/10.15414/2019.9788055220703

4 International Scientific Conference Abstracts Book
th
ANTIMICROBIAL AND SOME BIOCHEMICAL PROPERTIES OF
VACCINIUM VITIS‐IDAEA L.
Maryna Kryvtsova , Ivan Salamon , Jana Koščová , Jarmila Eftimova 3
3
2
1
1 Uzhhorod National University, Uzhhorod, Ukraine; E-mail: maryna.krivcova@gmail.com
2 University of Presov, Slovakia
3 University of Veterinary Medicine and Pharmacy in Košice, Slovakia

Over the past years, there has been a growing interest to vegetable raw materials
evaluated from the viewpoint of their potential antimicrobial activity. Vaccinium L. genus
plants are known to contain a whole spectrum of biologically active substances with anti-
inflammatory, antidiabetic, gene-protective, antioxidant and antimicrobial properties.
The purpose of the work was to study the antimicrobial, antioxidant and some
biochemical properties of alcoholic extracts of fruit and leaves of Vaccinium vitis‐idaea L.,
gathered in the Ukrainian Carpathians. The plants for the study were gathered around the
village of Pylypets, Mizhhiria district, Zakarpatska region (Transcarpathia). From the fruit and
leaves, ethyl and methyl extracts were produced. The subjects for the study were their
antioxidant activity (by DPPH method), total tannin and flavonoids (by spectrophotometric
method), and antimicrobial activity (by diffusion-into-agar method). For the purpose of study,
reference and clinical isolates were used from the oral cavity of human patients suffering from
inflammatory diseases of the periodontium, characterized by wide resistance spectrum to
antibiotics.
The studies have shown that the extracts displayed antimicrobial activity against Gram-
positive bacteria. Ethyl extract of Vaccinium vitis‐idaea fruit was established to display
antimicrobial activity against clinical and reference strains of S. aureus, B. subtilis, S. pyogenes.
Ethyl extract of leaves was seen to display a somewhat lower activity against S. aureus and S.
pyogenes. Similar trends were shown for methyl extract of leaves. Less expressed
antimicrobial activity was peculiar for methyl extract of the fruit.
The extracts were characterized by high antioxidant activity; the highest activity was
displayed by ethyl leaf and fruit extracts, somewhat lower – by methyl extracts. Out of all
extracts under review, it was the ethyl fruit extract that showed the highest level of tannins.
The highest level of flavonoids was registered in the ethyl leaf extract; their quantity in the
fruit extracts was 10 times lower. A low level of flavonoids was also registered in the methyl
extracts. The tannin contents of the leaves of ethanol and methanol extract equalled to 3.906
and 3.451 %, respectively. The lowest level of tannins was peculiar for the methanol fruit
extract.
Thus, the highest antibacterial activity was displayed by the ethyl extract of Vaccinium
vitis‐idaea It is worth noting that the extracts displayed antimicrobial activity against both
reference and clinical isolates of S. aureus, including methicillin-resistant ones. The ethyl fruit
extract and methanol leaf extract were established to show antimicrobial effect against S.
pyogenes. The ethanol fruit and leaf extracts and methanol fruit extract were observed to
show weak antimycotic activity. The fruit extracts were characterized by low antimycotic
activity. The established regularities cause good prospects for further studies of the use of
Vaccinium vitis‐idaea as a source of substances with antimicrobial activity against antibiotic-
resistant representatives of opportunistic microbiota.
Keywords: antimicrobial properties, antioxidant activity, Vaccinium vitis‐idaea L.

Acknowledgment
The study was supported by the project APVV-15-0377.

|44 4 International Scientific Conference Agrobiodiversity Nutrition, Health and Quality of Human and Bees Life
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September 11–13, 2019