Page 46 - Zbornik_Konf_2019

https://doi.org/10.15414/2019.9788055220703

4 International Scientific Conference Abstracts Book
th
EFFECT OF ENDOPHYTIC BACILLUS SUBTILIS AND SALICYLIC ACID ON RESISTANCE
OF SOLANUM TUBEROSUM L. (POTATO) TO POSTHARVEST DISEASES
Oksana Lastochkina , Liudmila Pusenkova , Andrey Baymiev ,
1,2
1
2,3
Irina Shpirnaya , Aysylu Shayahmetova , Darya Kulabuhova , Igor Koryakov , Svetlana
3
3
1
1
Garipova , Cemal Kasnak , Recep Palamutoglu 4
4
1,3
1 Bashkir Research Institute of Agriculture – Subdivision of the Ufa Federal Research Centre of the
Russian Academy of Sciences, Ufa, Russian Federation; E-mail.: oksanaibg@gmail.com
2 Institute of Biochemistry and Genetics – Subdivision of the Ufa Federal Research Centre of the Russian
Academy of Sciences, Ufa, Russian Federation
3 Bashkir State University, Ufa, Russian Federation
4 Afyonkarahisar Health Science University, Health Science Faculty, Department of Nutrition and
Dietetics, Afyon, Turkey
Postharvest diseases of potato lead to significant food and economic losses worldwide. In
this work we analyzed the effect of beneficial endophytic bacteria B. subtilis (strains 10-4 and
26D) both individually and in combinations with the natural signal molecule salicylic acid (SA) on
resistance of potato to diseases caused by Phytophthora infestans (late blight) and Fusarium
oxysporum (fusarium wilt and dry rot) during storage.
The experiments carried out on hydroponically grown potato (Solanum tuberosum L., Cv.
Bashkirsky) mini-tubers which before storage were infected by Ph. infestans (10 spores/mL) and
8
F. oxysporum (10 spores/mL) and then with B. subtilis (10-4, 26D) in wide range of
6
concentrations (0, 10 , 10 , 10 , 10 , 10 , 10 CFU/mL) both alone and in mixes with SA (0.05 mM),
6
8
7
5
4
3
and stored for 3-5 months at 3±1°C. All microbiological, molecular and physio-biochemical
parameters were assessed using classical and modern methods of investigations.
It was revealed the dose-dependent nature of B. subtilis (10-4, 26D) activity against diseases
of potato during storage both individually and in mixes with SA. The most effective
concentrations of B. subtilis in the suppression of both Ph. infestans and F. oxysporum are 10 8
CFU/mL (10-4 and 26D), 10 CFU/mL (10-4+SA) and 10 CFU/mL (26D+SA) were found. The
7
6
ability of B. subtilis (10-4, 26D) effectively penetrate and colonize the internal tubers’ tissues
when applied immediately prior to storage and the ability of SA accelerate these processes has
been proven. Application of B. subtilis 10-4, 26D and their mixes with SA significantly increased
the activity of amylase (AMY) inhibitors both in healthy and F. oxysporum-infected tubers during
storage. While in tubers infected by Ph. infestans the AMY inhibitors’ activity were almost zero in
the variants (control (without bacteria), 10-4 and 10-4+SA), in variants 26D and 26D+SA were
slightly increased. At the same time, B. subtilis (10-4, 26D) had practically no effect on the activity
of protease inhibitors or in some cases (26D upon infection Ph. infestans and 26D+SA upon
infection F. oxysporum) contributed to a slight decrease in their activity. Also, found that B. subtilis
10-4, 26D and their compositions with SA decreased pathogens-induced glycoalkaloids (GA)
(solanine and chaconine) accumulation and significantly increased ascorbic acid (AA) content
(especially when B. subtilis applied with SA) in both healthy and infected tubers during storage.
Thus, obtained results indicated that B. subtilis 10-4, 26D and their combinations with SA
effectively suppress the development of Ph. infestans and F. oxysporum in potato tubers during
storage prolonging shelf-life and positively impact on the quality of stored products.

Keywords: Endophytic B. subtilis, Solanum tuberosum L., Phytophthora infestans, Fusarium oxysporum,
resistance and quality parameters.

Acknowledgments
This work was supported by the Russian Science Foundation (Grant № 18-76-00031).

|46 4 International Scientific Conference Agrobiodiversity Nutrition, Health and Quality of Human and Bees Life
th
September 11–13, 2019