Page 104 - Zbornik_Konf_2019

https://doi.org/10.15414/2019.9788055220703

4 International Scientific Conference Abstracts Book
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BIOLOGICAL ACTION OF THE MICROELEMENTS CITRATES IN HONEY BEES BODY AND
THEIR PRODUCTS
Iryna Kovalchuk , Alla Pashchenko , Igor Kykish 1
2
1
1 Institute of Animal Biology NAAS, Lviv, Ukraine; E-mail.: irenakovalchuk@ukr.net
2 National University of Life and Environmental Sciences of Ukraine, Kyiv, Ukraine
The study of biological effects of microelements to the honey bee colony and their
interaction with other elements in the application of mineral additives will allow finding
optimal and safe methods for stimulating the resistance of the organism of bees and their
productivity.
In the first series, five groups of bee colonies were formed, three in each. The I group –
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conditions of stationary maintenance with saturated sugar syrup (SS) 0.3 l/week/colony, II
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group – experimental, under similar conditions of feeding with the addition of 2 mg of Ni in
the form of citrate to the SS, III group – addition to SS with citrate of Co in a dose of 2 mg;
rd
IV group – addition to the SS with citrate of Ni in a dose of 1 mg, V group – under similar
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conditions with the addition to the sugar syrup citrates of Co in a dose of 2 mg and Ni – 1 mg.
The second series included seven groups of honey bees: control (I) – the group received 2 ml
of 50 % SS per cage a day. Bees of II , III , IV , V , VI , and VII experimental groups, with
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SS, received a trace element, in different dilutions – (1: 3000); (1: 2000); (1: 1500); (1: 1000);
(1: 700); (1: 500). The application of Co and Ni citrates in the spring period led to decrease
content of Zn, Co (II and V ), Cu, Mg (V ), Se (IV ) in the tissues of the bee organism
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(p < 0.001 – p < 0.01) and the increase of Fe, Mg (II ), Mn (IV ), which confirms the
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phenomenon of antagonism and synergism between these minerals. A probable increase
content of Mg, Zn, Fe, Cu, Co and Ni in bee cells was established, with the highest indices of
these mineral substances in the group receiving Ni citrate at a dose of 1.0 mg/0.3L/week/
colony against the background of a decrease in the content of Se (p <0.001 – p <0.01). The
biological effect of the Co and Ni citrates in bees was characterized by the improved
nutritional value of honey and its preservation with increased proline content and
concentration of hydrogen ions. Complex feeding of honey bees by citrates of microelements
in high dilutions (1:3000; 1:2000; 1:1500 and 1:1000) with sugar syrup caused their
corrective effect on mineral metabolism and increase (p <0.05–0.02; p <0.01–0.001) content
of Co, Zn, Cu and Ni in homogenized tissues of the whole organism. It was established that in
tissues of the organism of bees of the VI –VII experimental groups that received the trace
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element complex in dilutions 1:700 and 1:500, there were like the directed reliable (р<0.02–
0.001) differences of the content of Co, Zn, Cu and Ni, however in the homogenized tissues of
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the whole organism of the bees VI –VII of the experimental group was most deposited Ni,
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the level of which in tissues increased on 6.69 and 4.01 mg/kg; (p <0.001) and Co
(3.70 mg/kg; p <0.001), but only in the homogenized tissues of the bees of the VII
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experimental group compared with the specimens of the tissues of the bees of the control
group (I ).
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The conducted studies have shown that both separate and joint addition of citrates of
trace elements to the honey bees in the spring period led to even-balanced changes in the
content of certain mineral elements in their tissues. Activating the physiological capabilities of
the honey bees by feeding organic salts of trace elements is a safe alternative that can be used
to improve the viability and productivity of bee colonies in certain seasonal periods.
Keywords: honeybees, microelements, Co citrates, Ni citrates.

|104 4 International Scientific Conference Agrobiodiversity Nutrition, Health and Quality of Human and Bees Life
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September 11–13, 2019