Page 103 - Zbornik_Konf_2019

https://doi.org/10.15414/2019.9788055220703

4 International Scientific Conference Abstracts Book
th
DGAT1 GENE POLYMORPHISM INFLUENCE ON A LACTIC PRODUCTIVITY OF UKRAINIAN
BLACK‐AND‐WHITE MILK BREED COWS
Svitlana Kostenko , Nataliya Svyrydenko , Ruslan Oblap , Nina Novak 2
1
1
2
1 National University of Life and Environmental Sciences of Ukraine,
Kyiv, Ukraine; E-mail.: natasvyrydenko@gmail.com
2 State Enterprise 'Ukrmetrteststandard', Kyiv, Ukraine

Lipid metabolism plays a leading role in the formation of dairy and meat productivity of
animals. It can influence the following attributes, that fat content in milk, slaughter quality of
animals, marbleness and tenderness of the meat, and the amount of hypodermic fat
(PCT/AU98/00882). Genes that determine the productive qualities of animals, one of which is
the DGAT1 gene, have been identified. In cattle, the gene DGAT1 is localized on the 14
chromosomes. The polymorphism of this gene is related to the fat content of cow`s milk and
it`s quality, as it participates in lipids synthesis (Grisart, et. al., 2002; Locarte, et al., 2006;
Shaydullin and Ganiev, 2017).
The purpose of our research was to study the effect of various genotypes of the large
cattle of Ukrainian black-and-white milk breed with gene DGAT1 on the milk productivity of
cows. The study was carried out on animals of Ukrainian black-and-white milk breed of cattle
at the NP NULES of Ukraine "Agronomic Experimental Station". Samples of venous blood were
obtained for analysis and PCR was performed.
Based on the results of the studies, three experimental groups were formed according to
the genotype of animals with DGAT1 gene: I group – cows with genotype AA, group II – AK and
group III – genotype of КК. For each group, the analysis of milk productivity indicators were
performed: milk yield for 305 days of lactation, fat content in milk, the quantity of milk fat.
Among the animals of the entire examined the population of Ukrainian black-and-white milk
breed for the DGAT1 genome, the genotype AA was found most frequently at 57 % of all cases,
AK-35 %, and KK in only 8 %.
The highest rates of lactation for 305 days were obtained from animals of I Group, for
the first lactation they produced 3380.35 kg of milk, the second – 46265.50 kg, and the third –
4189.42 kg, what is somewhat inferior to the yield level of cows, that belonged to groups III
and II. A similar advantage of heterozygous animals with genotype AK was observed for the
fat content in milk for the first lactation, however, with age (2nd and 3d lactation), this
indicator was slightly higher in cows with the genotype of CC – 3.68 % versus 3.59–3.64 and
3.61–3.67 % in analogs with genotypes AA and KK, respectively. However, it should be noted
that throughout the research period, the largest amount of milk fat was obtained from animals
of the second group, which, in our opinion, can be explained by the higher levels of these cows
yields. The difference in the average of yields compared to cows of the first and third groups
was 16.1 and 14.2 kg, respectively.
The results of our research comply with the data of Novak (2008), Oblap (2008), and
Shaidullin (2017), who concluded that animals with the genotype DGAT1 КК have a higher
percentage of fat in milk. As a result of the studies, it can be concluded that animals with the
genotype DGAT1 AK had the highest yields for 305 days of lactation and the highest amount of
milk fat. Cows with the genotype DGAT1 КК, were although somewhat inferior to the level of
the yield, but had a higher fat content in milk. The obtained data can be used for genotyping of
animals to this gene and improving the efficiency of breeding work in order to increase the
content of fat in milk.

Keywords: gene polymorphism, lactic productivity, fat content in milk.

4 International Scientific Conference Agrobiodiversity Nutrition, Health and Quality of Human and Bees Life |103
th
September 11–13, 2019