Page 107 - Zbornik_Konf_2019

https://doi.org/10.15414/2019.9788055220703

4 International Scientific Conference Abstracts Book
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ANTIBIOFILM‐FORMING ACTIVITY OF VACCINIUM VITIS‐IDAEA L.
LEAVES EXTRACT
Maryna Kryvtsova , Jana Koščová , Ján Király , Mykola Spivak 3
2
1
2
1 Uzhhorod National University, Uzhhorod, Ukraine; E-mail.: maryna.krivcova@gmail.com
2 University of Veterinary Medicine and Pharmacy in Košice, Slovakia
3 D.K. Zabolotny Institute of Microbiology and Virology of NAS of Ukraine, Kyiv, Ukraine

The continuously growing trend for the formation and circulation of antibiotic-resistant
strains of opportunistic pathogenic microorganisms calls forth the topicality of studies in
search of alternative means with antimicrobial properties. Development and introduction of
new antibacterial materials and approaches to treatment and correction of inflammatory
processes caused by opportunistic pathogenic microorganisms, and search for the
antibacterial materials that may in a number of cases become an alternative to antibiotic
treatment, also remains today an issue of primary importance. The ability of microorganisms
to form biofilm creates the prerequisites for the development of chronic inflammatory
processes. Under such conditions, search for alternative sources of antimicrobial activity
becomes a particularly pressing issue. Plant products that combine antimicrobial activity with
antioxidant, anti-inflammatory and deodorizing properties are a promising source for such
components.
Genus Vaccinium L. includes around 450 species found mostly in the northern
hemisphere of our planet. Fruit and leaves of these Vaccinium species produce a wide range of
compounds: flavonoids such as anthocyanins, flavonols, flavanols (catechins), phenolic acids
(benzoic and cinnamic acid derivatives), chromones, coumarins, lignans, iridoids, sterols, and
triterpenoids. The principal components are flavonoids (anthocyanins). Extracts of
anthocyanins from Vaccinium fruit and leaves demonstrate various pharmacological effects
such as antidiabetic, anti-inflammatory, vasoprotective, antimicrobial, antitumor, gene-
protective, and antioxidative
The purpose of the work was to study the antibiofilm-forming activity of ethanol leaves
extract of cowberry (Vaccinium vitis‐idaea L.), gathered in the Ukrainian Carpathians. From
the leaves, ethyl extract was produced. The antibiofilm activity of the EO was tested in
standard 96-well microtitration plates (Greiner-BioOne, Austria) using a modified staining
method according to O’ Toole (O’Toole, 2011). With the purpose of the study of the
antibiofilm-forming activity, an 18-hour culture of the reference S.aureus CCM 3953 biofilm-
forming strain grown at 37 C was used.
o
We have shown the antibiofilm-forming activity of ethanol Vaccinium vitis‐idaea leaves
extract. It has been established that biofilm destruction by 67.6 % was registered even when
affected with the lowest possible concentration of ethanol leaf extracts (0.01 %). The
introduction of a higher concentration of extracts (0.05 %) caused biofilm destruction by 68
%; of a 0.1 % concentration – by 73.4 %.
In our preceding works (Kryvtsova et all., 2019), we showed that extract of cowberry
fruit and leaves was established to possess a broad spectrum of antimicrobial activity against
Gram-positive, Gram-negative microorganisms and Candida genus fungi. The study of
antimicrobial, anti-biofilm forming, antioxidant and some biochemical properties let us
ascertain that extracts of Vaccinium vitis‐idaea leaves may exert high antimicrobial and anti-
biofilm forming effect even upon polyantibiotic-resistant microorganism strains of skin and
oral cavity.

Keywords: antibiofilm-forming activity, antimicrobial effect, Vaccinium vitis‐idaea L.
Acknowledgment
The study was supported by the project APVV-15-0377.

4 International Scientific Conference Agrobiodiversity Nutrition, Health and Quality of Human and Bees Life |107
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September 11–13, 2019