Page 109 - Zbornik_Konf_2019

https://doi.org/10.15414/2019.9788055220703

4 International Scientific Conference Abstracts Book
th
BIOLOGICAL ACTIVITY OF JUJUBE FRUITS (ZIZIPHUS JUJUBA MILL.)

Alicja Zofia Kucharska , Olga Grygorieva , Anna Sokół‐Łętowska , Svitlana Klymenko ,
1
2
2
1
Narcyz Piórecki 3,4
1 Department of Fruit, Vegetable and Plant Nutraceutical Technology, Wrocław University of
Environmental and Life Sciences, Wrocław, Poland; E-mail.: alicja.kucharska@upwr.edu.pl
2 M.M. Gryshko National Botanical Garden of the National Academy of Sciences of Ukraine,
Kyiv, Ukraine
3 Arboretum and Institute of Physiography in Bolestraszyce, Przemyśl, Poland
4 Department of Tourism and Recreation, University of Rzeszów, Poland
Ziziphus jujuba Mill. (jujube, Chinese date, Chinese jujube) is a species of Ziziphus Mill. in
the Rhamnaceae Juss. has been cultivated from ancient time in China for the last 5000 years.
Fruits, leaves, seeds and other parts of Ziziphus jujuba plant contain many important
biologically active substances. Therefore, their physiotherapeutic effects are used to treat
many diseases such as liver disease, fever, sore throat, pharyngitis, bronchitis, diabetes, skin
infections, loss of appetite, diarrhea. It is also used for digestive disorders, weakness, urinary
problems, and insomnia. It alleviates brain nerve disorders, regulates immune function and
it’s used to reduce blood triglycerides. It has anti-cancer, antifungal, antibacterial, anti-
inflammatory, hypotonic, antioxidant and immunostimulatory properties. The fruits contain
vitamin C, flavonoids, phenolic, amino acids, organic acids, polysaccharides, and
microelements.
The aim of this work was to evaluate the biological activity of fruits of Ziziphus jujuba
genotypes, as potential species for cultivation and use in Ukraine.
The objects of the research were 25-year-old plants of Ziziphus jujuba from seed origin,
which are growing in Forest-Steppe of Ukraine in M.M. Gryshko National Botanical Garden of
NAS of Ukraine (Kyiv).
The total antioxidant activity and the content of phenolic compounds and vitamin C in
the fruits (fresh weight) of the Ziziphus jujuba of 9 genotypes were compared. The raw
materials were collected in the season of full ripeness (October). Antioxidant activity (AOA)
was measured using three different photometric methods (DPPH – 2,2-diphenyl-1-
picrylhydrazyl, ABTS – 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid, FRAP – ferric-
reducing antioxidant power). Total polyphenol content (TPC) was evaluated using the Folin-
Ciocalteu reagent assay. Content of vitamin C was determined by using the titrimetric method
with 2,6-dichloroindophenol.
The results for AOA (μmol Trolox/g) determined by the DPPH method varied from
7.30±0.54 to 30.52±0.68, those obtained by the ABTS method varied from 19.65±0.30 to
59.29±3.87, and those obtained by the FRAP method varied from 9.04±0.96 to 24.33±2.18.
The results for TPC varied from 580.97±23.66 to 758.92±16.59 mg/100 g. Vitamin C content
ranged from 212.71±8.58 to 626.50±3.62 mg/100 g. A positive linear correlation was found
between antioxidant activity and total polyphenol content and vitamin C in the examined
plant material.
The results showed that all fruit extracts exhibited strong antioxidant activities, which
generally correlated positively with the total polyphenol content and vitamin C and
demonstrates the potential of the fruits as a possible source of valuable polyphenol content
and vitamin C, with high antioxidant activities and health-promoting properties.

Keywords: Ziziphus jujuba, fruits, antioxidant activity, polyphenol content, vitamin C.
Acknowledgments
The publication was prepared with the active participation of researchers in international network
AgroBioNet within the project ITMS 25110320104. The authors are grateful Bilateral Scholarship of
the Ministry of Education, Science, Research and Sport (Slovak Republic) and Polish National
Commission for UNESCO.

4 International Scientific Conference Agrobiodiversity Nutrition, Health and Quality of Human and Bees Life |109
th
September 11–13, 2019