Page 98 - Zbornik_Konf_2019

https://doi.org/10.15414/2019.9788055220703

4 International Scientific Conference Abstracts Book
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DETERMINATION OF ANTIOXIDANT CAPACITY AND POLYPHENOLS CONTENT IN FRUITS
OF CULTIVARS OF CHAENOMELES JAPONICA LINDL. EX SPACH.
Svitlana Klymenko , Alicja Zofia Kucharska , Narcyz Piórecki 3,4
2
1
1 M.M. Gryshko National Botanical Garden of the National Academy of Sciences of Ukraine, Kyiv,
Ukraine; E-mail.: cornusklymenko@gmail.com
2 Department of Fruit, Vegetable and Plant Nutraceutical Technology, Wrocław University of
Environmental and Life Sciences, Wrocław, Poland
3 Arboretum and Institute of Physiography in Bolestraszyce, Przemyśl, Poland
4 Department of Tourism and Recreation, University of Rzeszów, Poland
Consumer interest in health foods has increased market demand for high-quality fruits
such as the Chaenomeles spp. (flowering quince, Japanese quince). This fruit species posses
a significant potential for cultivation in monocultures as well. Chaenomeles cultivation is
slowly growing throughout the world as it delivers delicious and nutritious fruits. The genus
Chaenomeles Lindl. belongs to the Rosaceae Juss. family and comprises four species:
C. cathayensis (Hemsl.) C.K.Schneid., C. japonica (Thunb.) Lindl., C. speciosa (Sweet) Nak. and
C. thibetica T.T.Yu. Japanese quince (Chaenomeles japonica) is a shrub naturally occurring in
central and southern Japan. Compared to the other three species, Japanese quince is best
suited to the climate of northern Europe, where it was introduced in 1869. In traditional
Chinese medicine, the fruits of various species of Chaenomeles have been used for thousands
of years in the treatment of rheumatoid arthritis, hepatitis, asthma, and the common cold, and
also have been reported to have anti-inflammatory, antinociceptive, antimicrobial,
antioxidant, immunoregulatory, antitumor, hepatoprotective, antiparkinsonian, and
antioxidant activities. The fruits contain many bioactive components. Cold-pressed
Chaenomeles japonica seed oil is one of the richest sources of natural micro constituents, such
as fatty acids, carotenoids, squalene, polyphenols, phytosterols, tocopherols, and other
compounds with high biological activity.
The aim of this work was to evaluate the biological activity of fruits of Chaenomeles
japonica cultivars, as potential species for cultivation and use in Ukraine.
The objects of the research were 14 cultivars of Chaenomeles japonica the selection of
M.M. Gryshko National Botanical Garden of NAS of Ukraine (Kyiv). The raw materials were
collected in the season of full ripeness (October). Antioxidant activity (AOA) was measured
using three different photometric methods (DPPH – 2,2-diphenyl-1-picrylhydrazyl, ABTS –
2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid, FRAP – ferric-reducing antioxidant
power). Total polyphenol content (TPC) was evaluated using the Folin-Ciocalteu reagent
assay.
The results for AOA (μmol Trolox/g) determined by the DPPH method varied from
10.08±0.47 to 21.42±0.80, those obtained by the ABTS method varied from 31.13±4.66 to
70.87±4.60, and those obtained by the FRAP method varied from 14.97±0.78 to 42.51±1.80.
The results for TPC varied from 162.46±2.41 to 408.09±8.67 mg/100 g.
The results showed that all fruit extracts exhibited strong antioxidant activities, which
generally correlated positively with the total phenol contents. Our results point to the fact that
observed plants of cultivars Chaenomeles the selection of M.M. Gryshko National Botanical
Garden are suitable natural source of compounds, which have high antioxidant activity. This
means that the plants of Chaenomeles japonica may be used as a source of new health
resources when considering nutritional properties of the world’s less traditional fruit species.
Keywords: Chaenomeles japonica, cultivars, fruits, antioxidant activity, polyphenols contents.

|98 4 International Scientific Conference Agrobiodiversity Nutrition, Health and Quality of Human and Bees Life
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September 11–13, 2019