Page 58 - Zbornik_Konf_2019

https://doi.org/10.15414/2019.9788055220703

4 International Scientific Conference Abstracts Book
th
CELL CYCLE REGULATION AND PROAPOPTOTIC ACTIVITY OF
PHALLUS IMPUDICUS L.
Аnastasiia Shemediuk , Nataliya Shemedyuk 2
1
1 Ivan Franko National University of Lviv, Ukraine; Е-mail.: natshem13@gmail.com
2 Stepan Gzhytskyj National University of Veterinary Medicine and Biotechnologies,
Lviv, Ukraine

Apoptosis and cell cycle regulation are the main processes for maintaining the state
function of the cell. Studying in this sphere is actual because these processes determine the
exact reproduction of genetic information in daughter cells and eliminate cell transformation.
The programmed cell death is evidenced by the expression of genes responsible for the
synthesis of proteins involved in the biochemical cascade of apoptosis reactions, in particular,
caspase-3, p53, and the anti-apoptotic protein Bcl-2, the analysis of which involvement in
molecular mechanisms under the influence of Phallus impudicus L. extract on the cell cycle we
studied. In traditional medicine Ph. impudicus use for the treatment of different illnesses,
cardiovascular diseases, cancer, diseases of the reproductive system, during chemotherapy
and radiotherapy to prevent metastasis and recurrence oncological illness. Scientists research
separate biological active compounds involved in proapoptosis, anti-proliferative activities as
to cancer cells. As Ph. impudicus have wide variety of valuable pharmacological properties, it
is interesting to examine its toxicity, mechanisms interacting with cell's DNA.
The goal is to investigate the induction of apoptosis in GC‐1 spg, GC‐2 spd cells under the
influence of Ph. impudicus, studying the expression of proteins: caspase-3, p53, Bcl-2, changes
in the profile of the cell cycle. For research were used two mouse cell lines: spermatogonia
germ cells GC‐1 spg and spermatocyte cells GC‐2 spd under the influence of 3 % Ph. Impudicus
extract. Also, the object of research is gene expression in mentioned cells. For experiments,
cells were treated with 3 % Ph. impudicus extract prepared by direct dilution in the cell
culture medium. The cell cycle was profiled using DNA Cell cycle plug-in from ImageJ software
on fluorescent microphotographs taken on InCell Analyzer 2000. The protein expression was
examined by Western-Blot analyze.
The results of our studies are inhibition of the cell cycle of the 3 % Ph. impudicus extract
of cell lines in the G0/G1 phase. For the GC‐2 spg cell number of cell in G0/G1 phase increased
by 11.7 % and on the other side we have reduced the number of cells in the G2/M phase by
31.3 % relative to the controls. The GC‐1 spg cell line reacts similarly only with lower
numerical values: in the G0/G1 phase, the number of cells increases by 5.2 % due to
a decrease in the number of cells in the G2/M phase by 16.8 % relative to the controls.
Increased expression of caspase-3, p53 under the influence of extract after 96 hours of
incubation and reduction of Bcl-2 expression in both lines were noticed. This proves the
presence of current BAC in medicine at Ph. impudicus, the target of which is the DNA.
Analyzing the scientific data, it was investigated that BAC Ph. impudicus phloretin stops the
cell cycle in the G0 / G1 phase, induces expression of the proapoptotic proteins of caspase-3,
p53, inhibits the expression of the anti-apoptotic protein Bcl-2.
Under the influence of 3 % Ph. Impudicus extract cell cycle was blocked in GC‐1 spg and
GC‐2 spd cell lines in the G0 / G1 phase, enhancement of expression of apoptosis markers -
caspase-3, p53, inhibition of Bcl-2 was investigated.
Keywords: Phallus impudicus, cell cycle, apoptosis, biologically active compounds, spermatocytes,
spermatogonia.

|58 4 International Scientific Conference Agrobiodiversity Nutrition, Health and Quality of Human and Bees Life
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September 11–13, 2019