Page 27 - Zbornik_Konf_2019

https://doi.org/10.15414/2019.9788055220703

4 International Scientific Conference Abstracts Book
th
BIOLOGICAL ACTIVITY OF EXTRACTS FROM SOME SPECIES OF CONIFEROUS PLANTS

Dina Elisovetcaia , Raisa Ivanova , Diana Gladei , Jana Simkova , Ján Brindza
1
1
2
2
1
1 Institute of Genetics, Physiology and Plant Protection, Chisinau, Republic of Moldova;
E-mail.: dina.elis.s@gmail.com
2 Institute of Biological Conservation and Biosafety, Slovak University of Agriculture in Nitra, Slovak
Republic
The development and use of biological preparations based on plant secondary
metabolites is an important trend in modern organic farming. The aim of this study was to
determine the biological activity of extracts and essential oils from some species of Coniferous
plants, namely Juniperus sabina L. and Pinus sylvestris L., growing in Republic of Moldova (RM)
and in Slovak Republic – Nitra (SN) and Pianiny mountains (PM).
The antioxidant activity of ethanolic extracts of Coniferous was determined by two
procedures with appreciation of their radical scavenging capacities against DPPH and peroxyl
free radicals. The trolox and gallic acid serve as standard antioxidant substances. The
antioxidant activity of plant extracts was evaluated in equivalent of both standard substances
and by indexes IC50 (the extract concentration that scavenged 50 % of free radicals).
According to all determined indexes the antioxidant activity of tested extracts from
Coniferous plants was qualified in followed order: J. sabina SN ˃ J. sabina PM > J. sabina RM ˃
P. sylvestris RM ≥ P. sylvestris SN. It should be mention that the scavenging of the same amount
of peroxyl radicals was carried out with the lower concentrations of alcoholic extracts than to
inhibit of DPPH radicals. Therefore, the indexes IC50 (mg per g of dry residue) of extracts
against DPPH and peroxyl free radicals were equal, respectively: 1.64±0.01 and 0.13±0.01 for
J. sabina SN; 1.93±0.01 and 0.15±0.04 for J. sabina PM; 2.60±0.17 and 0.16±0.02 for J. sabina
RM; 6.68±0.64 and 0.41±0.03 for P. sylvestris RM; 7.28±0.55 and 0.51±0.04 for P. sylvestris SN.
Moreover, the biopesticidal (insecticidal, ovicidal, antifeedant, repellent and deterrent)
properties of Coniferous plant extracts and oils were studied. As a result of biological activity
testing it was established that both ethanolic extracts and essential oil J. sabina possessed high
efficiency against Colorado Potato beetle Leptinotarsa decemlineata Say and wax moth
Galleria mellonella L. Mortality of imago and larvae consisted in average 6.7–53.3 and 66.7–
100 % respectively, ovicidal activity was 100 %, and antifeedant effect persisted at the level of
1–3 points for imago and 1 point for larvae. It was established that the treatment of feed and
insects with the essential oils of P. sylvestris needles resulted in the death of only 6.7–13.3 %
of the wax moth larvae. There was also noted a decrease at the rate of formed pupae and
emergence imagoes by 20 % compared with the control. It was determined that essential oils
had a deterrent effect on egg-laying of the wax moth.
Extracts from J. sabina exhibited a selective effect on various groups of insects and,
therefore, allow preserving the biodiversity of agrocoenosis, which is favorable for
entomophagous. The results will serve as a basis for the selection of the most effective plant
extracts for further their use to protect plants from pests, as well as to increase the resistance
of plants to diseases and other adverse environmental factors.

Keywords: Coniferous plant, extract, essential oil, antioxidant activity, biopesticide property.
Acknowledgments
The authors are grateful to the International Visegrad Fund (Bratislava) for supporting the research
internship project (51810070) and project BIOFOOD ITMS 25 110 320 104.

4 International Scientific Conference Agrobiodiversity Nutrition, Health and Quality of Human and Bees Life |27
th
September 11–13, 2019