Page 148 - Zbornik_Konf_2019

https://doi.org/10.15414/2019.9788055220703

4 International Scientific Conference Abstracts Book
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CONTENT OF PHOTOSYNTHETIC PIGMENTS IN THE LEAVES OF CRAMBE L. SPECIES

Olena Vergun, Oksana Shymanska, Dzhamal Rakhmetov, Valentyna Fishchenko,
Oleksandr Bondarchuk, Svitlana Rakhmetova

M.M. Gryshko National Botanical Garden of the NAS of Ukraine, Kyiv, Ukraine;
E-mail.: en_vergun@ukr.net
Pigment complex is an important parameter in the ecological study and it is a marker of
plant condition related to the environment. In this case, the most used parameters are the
content of chlorophylls, carotenoids, and their ratio. The previous study of Crambe abissinica
Hochst showed that exists a correlation between the content of nitrogen and chlorophylls in
the leaves.
In this study, we used to investigate the content of chlorophyll a (Chl a), b (Chl b),
carotenoids (car) and their ratio in four species of Crambe L. during vegetation: C. cordifolia
Steven, C. koktebelica (Junge) N.Busch, C. maritima L., C. steveniana Rupr. collected in the M.M.
Gryshko National Botanical Garden of the NAS of Ukraine (Kyjiv). Middle height increment of
investigated plants between stages of growth given. The concentration of chlorophylls a, b
and car measured spectrophotometrically on UNICO 2800 at the wavelength 662, 644 and
440, respectively. Extraction procedure conducted in acetone (Musienko et al., 2001).
Obtained results are given in mg/g of fresh mass.
The height increment in a period of spring vegetation-budding was 26.3–80.2 cm,
budding-flowering 12.3–136.0 cm, flowering-fruitage 1.3–16.6 cm depending on species. C.
cordifolia had the most value of this parameter at the budding–flowering period and C.
maritima had the least value at the flowering–fruitage period.
Content of chlorophyll a at the spring vegetation period was 0.321–0.490 mg/g, at the
budding period 0.237–0.680 mg/g, at the flowering period 0.349–0.680 mg/g and at the
fruitage 0.478–0.962 mg/g depending on species. Content of chlorophyll b was 0.133–0.203
mg/g at the spring vegetation stage, 0.158–0.275 mg/g at the budding stage, 0.158–0.275
mg/g at the flowering stage and 0.227–0.439 mg/g at the fruitage depending on species. The
concentration of carotenoids was 0.038–0.078 mg/g at the spring vegetation period, 0.043–
0.104 mg/g at the budding period, 0.070–0.201 mg/g at the flowering period and 0.117–0.435
mg/g at the fruitage depending on species.
In the start of vegetation between process of grow and accumulation of photosynthetic
pigments exists the correlation. So, a correlation between the content of Chl a and increment
of plants was strong at the spring vegetation (r = 0.78), moderate at the budding period (r =
0.52) and strong at the flowering (r = 0.76). At the flowering stage found a very strong
correlation (r = 0.90) between Chl b content and increment. Very strong and strong
correlation found between increment and car content at the spring vegetation (r = 0.84) and
budding period (r = 0.69), respectively. Also, a very strong correlation identified between the
increment of plants and the ratio of chlorophylls (r = 0.89) at the end of vegetation.
Thus, obtained data showed that between the accumulation of photosynthetic pigments
of investigated plants and increment of plants exists positive correlation, which depends on
the period of growing. Further, it’s can be used for the study of total productivity and some
grow parameters of these plants.

Keywords: Crambe, chlorophyll a, chlorophyll b, carotenoids.
Acknowledgments
Authors are grateful for scientific net AgroBioNet for the supporting of this study.

|148 4 International Scientific Conference Agrobiodiversity Nutrition, Health and Quality of Human and Bees Life
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September 11–13, 2019