Page 143 - Zbornik_Konf_2019

https://doi.org/10.15414/2019.9788055220703

4 International Scientific Conference Abstracts Book
th
CONTENT OF POLYPHENOL COMPOUNDS IN ETHANOL EXTRACTS OF
NEPETA GRANDIFLORA M. BIEB.
Ludmyla Svydenko , Olena Vergun , Zuzana Schubertová , Ján Brindza ,
2
3
1
3
Jan Mareček , Eva Ivanišová 4
4
1 Experimental Facility “Novokakhovska” of Rice Research Institute of Ukrainian Academy of
Agrarian Sciences, Plodove, Ukraine; E-mail: svid65@ukr.net
2 M.M. Gryshko National Botanical Garden of the NAS of Ukraine, Kyiv, Ukraine
3 Institute of Biological Conservation and Biosafety, Slovak University of Agriculture in Nitra,
Slovak Republic
4 Department of Technology and Quality of Plant Products, Slovak University of Agriculture in Nitra,
Slovak Republic
Plants of Nepeta L. widely known as a source of biologically active compounds and use in
folk medicine due to pharmacological and biological activities. These plants distributed in
Central and South Asia, Europe, Middle East, etc. These herbs have been used in folk medicine
to treat different disorders. Raw of Nepeta herbs exhibit such biological activities as an anti-
inflammatory, analgesic, antinociceptive, anti-atherosclerotic, antimicrobial, antifungal,
antiviral and repellent.
In this study investigated the antioxidant activity of Nepeta grandiflora M.Bieb.
harvested from the experimental collection of Experimental Facility “Novokakhovska” of Rice
Research Institute of Ukrainian Academy of Agrarian Sciences, v. Plodove at the both
flowering and fruitage periods. All biochemical analyses conducted in the Slovak University of
Agriculture in Nitra. It was determined total content of polyphenols (expressed in gallic acid
equivalent (GAE)), flavonoids (expressed in quercetin equivalent (QE)), phenolic acids
(expressed in caffeic acid equivalent (CAE)), antioxidant activity (expressed in Trolox
equivalent (TE)) and reducing power (expressed in Trolox equivalent (TE)) of extracts. All
measurements carried out on the spectrophotometer Jenway (6405 UV/Vis, England).
We identified that content of polyphenols among investigated plant extracts was from
21.84 (fruits; fruitage period) to 63.73 (leaves; flowering period) mg GAE/g of DW (dry
weight). The content of flavonoids was from 10.08 (fruits; fruitage period) to 34.07 (leaves;
flowering period) mg QE/g of DW. Content of phenolic acids of alcohol extracts was from 5.48
(fruits; fruitage period) to 27.97 (inflorescences; flowering period) mg CAE/g of DW.
Antioxidant activity by DPPH-method was from 8.54 (leaves; fruitage period) to 9.05 (fruits;
fruitage period) mg TE/g of DW. Reducing power of investigated extracts was from 18.60
(fruits; fruitage period) to 134.29 (inflorescences; flowering period) mg TE/g of DW. It was
found a very strong correlation between reducing power of extracts and content of
polyphenols, phenolic acids, flavonoids as 0.98, 0.96 and 0.84 respectively.
Thus, this study demonstrated that plants of N. grandiflora are a rich source of
antioxidant compounds possessing the antioxidant activity such as polyphenols, flavonoids,
and phenolic acids, which are correlated with reducing power of extracts by
phosphomolybdenum method. Along with other species of this genus, N. grandiflora can be
used in the pharmacological study.

Keywords: Nepeta grandiflora, polyphenols, phenolic acids, flavonoids, antioxidant activity.
Acknowledgments
Authors are grateful for the Visegrad Fund Scholarship Program (51810415) and National Scholarship
Programme of the Slovak Republic for supporting this research. This study also conducted within the
framework of AgroBioNet and project BIOFOOD ITMS 25 110 320 104.

4 International Scientific Conference Agrobiodiversity Nutrition, Health and Quality of Human and Bees Life |143
th
September 11–13, 2019