Page 147 - Zbornik_Konf_2019

https://doi.org/10.15414/2019.9788055220703

4 International Scientific Conference Abstracts Book
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BIOCHEMICAL COMPOSITION OF FOUR SPECIES OF CRAMBE L.

Olena Vergun, Oksana Shymanska, Dzhamal Rakhmetov, Valentyna Fishchenko,
Oleksandr Bondarchuk, Svitlana Rakhmetova

M.M. Gryshko National Botanical Garden of the NAS of Ukraine, Kyiv, Ukraine;
E-mail.: en_vergun@ukr.net
Species of plants of Crambe L. belong to Brassicaceae Burnett and their number achieve
up to 40. Last time investigations of these plants focused on their biochemical composition
especially oil content and energetic value. Plants of Crambe have economic interest also
because of low damage for soil quality, characterized by drought-resistance. Investigation of
accumulation of different compounds in above-ground part of these plants, also, an important
aspect for evaluation of perspective of use. The aim of this study was to compare the
peculiarities of the biochemical composition of Crambe species dynamically. In addition, our
previous investigation identified the high antioxidant activity of different extracts of
investigation plants.
Plant material collected from the experimental collection of M.M. Gryshko National
Botanical Garden of the NAS of Ukraine. It was studied above-ground parts of C. cordifolia
Steven, C. koktebelica (Junge) N.Busch, C. maritima L., C. steveniana Rupr. At the spring
vegetation, budding stage, flowering, and fruitage. Following biochemical parameters was
studied: dry matter by drying to consist weight at the 105 °C (Ermakov, 1972); content of
sugars by Bertrand's method using of glucose scale, ascorbic acids with 2.6-
dichlorophenolindophenol, tannins with indigo carmine discoloration, organic acids by
sodium hydroxide titration with phenolphthalein (Krishchenko, 1983), carotene with gasoline
galosh spectrophotometrically (Pleshkov, 1985), ash in muffle over (Hrycaenko et al., 2003).
We identified that during vegetation the dry matter was from 9.76 (C. cordifolia,
budding) to 22.54 (C. maritime at the fruitage) %, total content of sugars from 6.54 (C.
maritime at the fruitage) to 33.18 (C. cordifolia at the budding) %, ascorbic acid from 139.85
(C. maritime at the spring vegetation) to 987.02 (C. maritime at the budding) mg%, carotene
from 0.39 (C. maritime at the budding) to 1.82 (C. cordifolia at the fruitage) mg%, tannins from
1.28 (C. cordifolia at the fruitage) to 7.47 (C. steveniana at the budding) %, content of organic
acids from 3.12 (C. steveniana at the fruitage) to 6.28 (C. koktebelica at the spring vegetation)
%, ash from 6.11 (C. maritime at the spring vegetation) to 14.61 (C. cordifolia at the flowering)
%. Correlation analysis showed that between the content of tannins and organic acids existed
positive moderate correlation (r = 0.51). Between other parameters found the weak, very
weak positive or negative correlation.
It’s should be noted that the accumulation of nutrients in the above-ground part of
investigated plants was uneven. This depends on species and stage of grow.
Thus, comparing biochemical analyze identified that raw of four species of Crambe is a
valuable source of nutrients during vegetation. The high content of sugars, ascorbic acid, and
carotene can recommend these plants as forage crops. Also, raw of these plants can be
recommended for farther pharmacological investigations.
Keywords: Crambe, biochemical composition, raw.
Acknowledgments
Authors are grateful for scientific net AgroBioNet for the supporting of this study.

4 International Scientific Conference Agrobiodiversity Nutrition, Health and Quality of Human and Bees Life |147
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September 11–13, 2019