Page 139 - Zbornik_Konf_2019

https://doi.org/10.15414/2019.9788055220703

4 International Scientific Conference Abstracts Book
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SCREENING OF WHEAT CULTIVARS WITH EFFECTIVE RESISTANCE GENES TO THE HIGH
VIRULENT STRAIN OF STEM RUST UG99
Svitlana Spivak, Yaroslav Pirko, Nataliya Kozub, Anastasiia Rabokon, Anatoliy Karelov,
Bogdan Ivaschuk, Alla Yemets, Yaroslav Blume

Institute of Food Biotechnology and Genomics of the NAS of Ukraine,
Kyiv, Ukraine; E-mail.: svetlana_spivak@ukr.net

Stem rust is a dangerous disease of such important cereals as wheat and barley, caused
by Puccinia graminis f. sp. tritici. Among a large number of strains of this fungus, Ug99 is
known as most devastating. Ug99 strain demonstrates high dynamics of distribution during
the last decades and the ability to mutate. Main wheat stem rust resistance genes are not
efficient against this strain. There are some foreign wheat varieties which provide resistance
to Ug99, but their direct use is not possible in Ukraine because of agrotechnical
characteristics. Therefore, it is very important to create the own Ukrainian wheat varieties
with resistance to Ug99.
The main purpose of this work was to select the molecular-genetic markers for the
detection resistance genes to Ug99 strain of stem rust and the selection of foreign wheat
lines/varieties with effective resistance genes to strain Ug99 for further transfer and
pyramiding to modern Ukrainian wheat cultivars. As a result, the list of molecular-genetic
markers for effective resistance genes to the Ug99 strain (namely Sr2, Sr33, Sr39, Sr40 genes)
has been generated. This list included the nucleotide primer sequences, the annealing
temperature, the length of the expected amplicon, etc. Also, some of them can be used in the
study of the allelic state of resistance genes, for example csSr2, Sr39 # 22r, Sr39 # 50s,
Wmc344, Gwm374.
The presence of resistance genes to Ug99 in domestic and foreign breeding wheat
cultivars was determined using PCR. Genomic DNA from wheat seedlings was obtained using
the CTAB method. After PCR amplification the products were analyzed on a non-denaturing
acrylamide gel. A list of wheat cultivars of national and foreign breeding with resistance genes
to the Ug99 that can be used for crossings was created. It consisted of 16 samples: (RL5711 x
W984-8767), (RL6087 x W984-8767), DH31, (RL5711 x FL62R1), RL6087, RL5711, (RL6087
x Hoffman), Selkirk, DK20, (RL5711 x Carberry), (RL6087 x Carberry), Pembina, НК16 – the
country of origin is Canada; Renown – the country of origin is United Kingdom; Kharkivska 6,
Kharkivska 12 – the country of origin is Ukraine. In total among 16 tested specimens, it was
found 13 which are characterized by the presence of resistant alleles of one or another Sr
gene (Sr 2, Sr 33, Sr 39, Sr 40). Among the varieties of Ukrainian breeding (Kharkivska 6 and
Kharkivska 12), involved in the study, none resistance alleles of the studied Sr genes was
showed.
Keywords: wheat, stem rust, strain Ug99, resistance genes, PCR analysis.

Acknowledgments: This work was particularly supported by the project of Ministry of Education and
Science of Ukraine “Creation of selection lines of soft wheat with resistance genes to stem rust strain
Ug99” (0117U006842).

4 International Scientific Conference Agrobiodiversity Nutrition, Health and Quality of Human and Bees Life |139
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September 11–13, 2019