Page 131 - Zbornik_Konf_2019

https://doi.org/10.15414/2019.9788055220703

4 International Scientific Conference Abstracts Book
th
EVALUATION OF MORPHOLOGICAL AND MOLECULAR MARKERS
OF FLAX (LINUM spp.) GERMPLASM COLLECTION
Katarína Ražná , Lucia Hlavačková, Janka Nožková , Nina Brutch
1
1
2
1 Department of Genetics and Plant Breeding, Faculty of Agrobiology and Food Resources, Slovak
University of Agriculture in Nitra, Slovak Republic; E-mail.: katarina.razna@uniag.sk
2 N.I. Vavilov Research Institute of Plant Industry, St. Petersburg, Russia

Newly discovered type of functional markers based on microRNA molecules represents
highly efficient, stable, reproducible, low cost and transferable genotyping technique. miRNAs
are types of endogenous, conserved, single-stranded, non-coding, 20–25 nt long RNAs with an
important regulatory role in plants development and growth. In this research, we focused on
the possibility of the interconnection of molecular markers based on miRNA and selected
morphological traits.
The variability of the morphological features of flax was evaluated according to the
classifier of Nôžková et al. (2011). Genotyping was performed in two replicates. Mechanically
undamaged and healthy plants were evaluated in each repetition. The results include image
documentation (altogether about 3500 images) at the level of flowers, capsules, in selected
phenological phases and after harvesting. Genomic fingerprinting of flax genotypes of
different economic type and origin were realized by miRNA markers by the touch-down PCR
method.
By using of 6 miRNA markers pairs: gm-miR156b-F/gm-miR-R, lus-miR168-F/lus-miR-
R, gm-miR156b-F/gm-miR171a-F, hyp-miR156a-F/hyp-miR414-F, gm-miR171a-F/lus-
miR168-F and gm-miR156b-F/lus-miR168-F we confirmed polymorphism and cross-species
transferability of conserved miRNA sequences. To distinguish oily genotypes from fiber and
wild genotypes the gm-miR156b-F/gm-miR-R marker combination was the most suitable.
Unique miRNA loci were amplified by each combination in oily genotype from Ethiopia.
Genotypes had the same manifestation in selected morphological traits irrespective of the
type and place of origin of the genotype.
Based on the results of the molecular-morphological analysis, unique genotypes
originated from Ethiopia and India was identified. Multi-level character classification, of the
flower characters, showed that genotypes originating from China, Morocco, and Afghanistan
had the same expression and genotypes from India and Ethiopia unique expression in all
three observed traits. Genotypes originating in Tunisia and Portugal had a consistent
expression in the flower bud color.
By molecular-morphological markers was found, that genotype k-6392 differed in the
variability of the number of loci and in the unique expression of traits associated with flowers,
from the other tested genotypes.

Keywords: Linum spp., miRNAs, molecular markers, morphological markers.
Acknowledgments
This research was supported by the project of European Community no. 26220220180: Building
Research Centre 'AgroBioTech'.

4 International Scientific Conference Agrobiodiversity Nutrition, Health and Quality of Human and Bees Life |131
th
September 11–13, 2019