Page 130 - Zbornik_Konf_2019

https://doi.org/10.15414/2019.9788055220703

4 International Scientific Conference Abstracts Book
th
REACTIONS OF POLLEN OF PLANTS FROM GENUS LYSIMACHIA L. ON DIFERRENT
ENVIROMENTAL CONDITIONS
Volodimir Radchenko, Raisa Matyashuk, Marina Mazura, Mikola Yurchuk

Institute for Evolutionary Ecology of NAS of Ukraine, Kyiv, Ukraine;
E-mail.: raisakiev2015@gmail.com

A cytological study of pollen of different species of the genus Lysimachia L. has been
carried out. This is part of the comprehensive study of biological, anatomical, cytological,
biochemical and molecular genetic features of the common species in Ukraine of this genus.
The genetic collection of the genus Lysimachia has been begun to create on the territory of
Feofania Park in Kyiv which is the government designated park. Representatives of this genus
are potentially important species for the purposes of pharmacology and to enrich the
biodiversity and efficient use of natural resources. The general appearance and sculpture of
pollen grains and the sensitivity of pollen of some species of the genus Lysimachia to different
conditions of plant cultivation were studied.
The pollen morphology and structure of his shell in of Lysimachia nummularia L.,
Lysimachia punctata L. and Lysimachia vulgaris L. studied using the scanning electron
microscope (JCM-600 Neoscope, IEOL Ltd). The obtained results made it possible to confirm
that the shape of pollen grains, the structure of its shell are genetically determined and bear
the specific features of the taxon. Only the size of pollen grains L. nummularia is slightly
smaller (length 30–32 microns, width 12–13 microns) than L. punctata (length 32–34
microns, width 17–18 microns).
Growth and development of plants of Lysimachia in different conditions of the
environment affect the biometric indices of pollen and its fertility. The sensitivity of pollen to
exogenous conditions is elevated during the flowering of plants, compared to the phase of
budding. There was a tendency towards a decrease in the diameter of pollen grains with the
flower opening of the L. punctata and L. vulgaris in the cultivation of plants in conditions with
increased exposure to anthropogenic factors. In L. nummularia with the flower opening, the
growth of pollen grains continues. Also, a high level of pollen fertility for plants of this species
has been established. The fertility of their pollen in the closed ripe bud is more than 90–92 %.
In less favorable conditions, plant growth fertility decreases to 73–75 %. Although L.
nummularia has very low productivity of fruit production and the formation of high-grade
seeds in the cultivation of plants in different ecological and climatic conditions.
Similarly, the high natural fertility potential of pollen (80–95 %) in the plant budding
phase was noted for L. punctata. But there is an active loss of it in the flowering phase when
growing plants under anthropogenic loading.
The fertility of the pollen of L. vulgaris during cultivation of the plant in favorable
conditions also reaches 80–90 %. The pollen of these plants is much larger when grown in the
meadow and synanthropic communities than in urban locality. The sensitivity of the pollen of
this species to the influence of exogenous factors increases significantly with the onset of
flowering of plants. Thus, the high living potential of the pollen of investigated species of the
genus Lysimachia and their sensitivity to growing conditions are established.

Key words: Lysimachia L., pollen, sensitivity.
Acknowledgments
The research was carried out within the framework of the target integrated multidisciplinary research
program of the National Academy of Sciences of Ukraine 'Molecular and cellular biotechnology for the
needs of medicine, industry and agriculture'.

|130 4 International Scientific Conference Agrobiodiversity Nutrition, Health and Quality of Human and Bees Life
th
September 11–13, 2019