Page 135 - Zbornik_Konf_2019

https://doi.org/10.15414/2019.9788055220703

4 International Scientific Conference Abstracts Book
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BIOLOGICAL ACTIVITY OF ETHANOL EXTRACTS OF GALEGA OFFICINALIS L.

Oksana Shymanska , Olena Vergun , Miroslava Kačániová , Ján Brindza ,
1
1
2
3
Dzhamal Rakhmetov , Eva Ivanišová 4
1
1 M.M. Gryshko National Botanical Garden of the NAS of Ukraine, Kyiv, Ukraine;
E-mail.: en_vergun@ukr.net
2 Department of Microbiology, Slovak University of Agriculture in Nitra, Slovak Republic
3 Institute of Biological Conservation and Biosafety, Slovak University of Agriculture in Nitra,
Slovak Republic
4 Department of Technology and Quality of Plant Products, Slovak University of Agriculture in Nitra,
Slovak Republic
Plants of Galega officinalis L. are known as forage, energetic and especially as medicinal
plants. It is a source of biologically active compounds that play an important role in regulation
and decreasing of sugar content in human blood. G. officinalis herb exhibits many biological
activities such as an anti-inflammatory, antioxidant, antimicrobial, antifungal, hypoglycemic,
diuretic, etc.
In this study investigated the antioxidant and microbiological activities of G. officinalis
(Fabaceae Lindl.) harvested from the experimental collection of M.M. Gryshko Botanical
Garden at both spring growth and flowering periods (above-ground part and root). All
analyses conducted in the Slovak University of Agriculture in Nitra. It was determined total
content of polyphenols, flavonoids, phenolic acids, antioxidant activity and reducing power of
extracts. It was used 9 microbial strains and disc diffusion method for the microbiological
study.
We identified that maximal values found for above-ground raw and minimal for roots.
Content of polyphenols in the period of spring vegetation and flowering of above-ground part
was 53.17 and 62.47 mg GAE/g DW (dry weight), respectively; flavonoids 33.69 and 27.38 mg
QE/g DW, respectively; phenolic acids 14.38 and 18.59 mg CAE/g DW, respectively;
antioxidant activity by DPPH-method 4.44 and 8.37 mg TE/g DW, respectively and reducing
power of investigated extracts was 134.29 and 177.38 mg TE/g DW, respectively. Content of
polyphenols in the period of spring vegetation and flowering of roots was 5.27 and 6.87 mg
GAE/g DW (dry weight), respectively; flavonoids 0.91 and 1.03 mg QE/g DW, respectively;
phenolic acids 2.42 and 3.20 mg CAE/g DW, respectively and reducing power of investigated
extracts was 90.13 and 115.20 mg TE/g DW, respectively. Antioxidant activity by DPPH-
method in the roots not detected.
Antimicrobial activity of roots extracts was higher than in above-ground extracts.
Inhibition zone was from 1.00 (against Candida tropicalis) to 5.00 (Salmonella enterica) mm
for roots extracts at the spring growth and from 1.50 (against C. tropicalis) to 3.67 (against
Staphylococcus aureus) mm in roots at the flowering period. Inhibition zone 1 mm was
detected against Salmonella enterica, Klebsiella pneumoniae, and Candida glabrata only at the
period of spring growth. Against other microbial strains, extracts were ineffective. In a period
of flowering inhibition zone detected from 1.17 (C. glabrata) to 1.50 (S. aureus, Candida
albicans, Salmonella enterica) mm.
Thus, this study demonstrated that plants of G. officinalis are a rich source of antioxidant
compounds. Antimicrobial activity was better in roots extracts. The results can be used in
farther pharmacological investigations with different concentrations.
Keywords: Galega officinalis, antioxidant properties, polyphenols, antimicrobial activity.

Acknoledgements
Authors are grateful for scientific net AgroBioNet for the supporting of this study.

4 International Scientific Conference Agrobiodiversity Nutrition, Health and Quality of Human and Bees Life |135
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September 11–13, 2019