Page 129 - Zbornik_Konf_2019

https://doi.org/10.15414/2019.9788055220703

4 International Scientific Conference Abstracts Book
th
CRAMBE GRANDIFLORA DC. PLANTS IN VITRO PROPAGATION

NadiaPushkarova , MykolaKuchuk , YaroslavBlume 1

2
1
1 Institute of Food Biotechnology and Genomics of NAS, Department of Cell Biology and Biotechnology,
Kyiv, Ukraine; E-mail.: nadu4ka@gmail.com
2 Institute of Cell Biology and Genetic Engineering of NAS, Department of Genetic Engineering, Kyiv,
Ukraine
Crambe grandiflora DC. is an endangered species with high potential for breeding and
cultivation as an oilseed, agricultural and technical plant. Due to the limited population’s
number and protected status biotechnological approaches for plant propagation should be
considered. The aim of the presented work was to elaborate efficient in vitro propagation
protocols and obtaining different types of an aseptic culture of C. grandiflora.
C. grandiflora aseptic petiole and leaf explants were used for morphogenic potential and
propagation scheme establishment. Explants were cultivated on MS (Murashige and Scoog)
medium supplemented with 1 to 5 mg/L of 6-benzylaminopurine (BA) or kinetin and with 0.1
to 1.5 mg/L of 1-naphthaleneacetic acid (NAA) growth regulators in different combinations.
For each type of explants, regeneration frequency was estimated. Plantlets were regenerated
from petiole explants on every media with BA and NAA that was tested though with different
frequency, but cultivation with kinetin and NAA was less successful. With low BA content
regeneration frequency was up to 80%. Cultivation on the medium with higher BA
concentration (2.5 to 5 mg/L) led to an increase in regeneration frequency up to 100 %. A
relation between NAA content (combined with BA) in the medium and regeneration
frequency was noted and its optimal level was 0.5 to 1 mg/L.
Leaf explants cultivation on medium with BA and NAA resulted in lower regeneration
frequency compared to petiole explants. After BA content increase up to 5 mg/L it was
possible to obtain the maximum of 80 % regeneration. On petiole explants callus tissue
formed in response to cultivation with BA and NAA from all over the explant and was more
than 10 mm wide though on leaf explants callus formed insignificantly and only on the cut
ends.
For petiole explants were grown on medium with kinetin and NAA the highest
regeneration frequency was observed with relatively low concentrations of kinetin in the
medium (1 to 2.5 mg/L). Further increase of kinetin concentration caused lower regeneration
frequency. For leaf explants grown on medium with kinetin the same tendency occurred –
higher regeneration in response to low kinetin content in the medium. It should be noted that
kinetin presence in the medium led to rhizogenesis and practically no callogenesis (only on
cut ends) from leaf explants as well as from petioles.
Therefore, the results of presented work are recommendations on the growth regulators
content in the medium for fast propagation of C. grandiflora plants in vitro: for petiole
explants – MS with addition of BA 2.5 and NAA 1 mg/L or BA 5 mg/L and NAA 0.5 mg/L and
for leaf explants – MS with kinetin 1 and NAA 1.5 mg/L.
Crambe graniflora plants have great potential as a source of biofuel and have a need for
protection and propagation measures. Therefore, we established aseptic plants culture and
developed recommendations for its fast propagation in vitro. Propagation via petiole and leaf
explants is possible but for petiole explants, the cultural medium should be supplemented
with kinetin and NAA and for petiole explants with BA and NAA.

Keywords: Crambe grandiflora, in vitro cultivation, oilseed, rare plants, plant propagation.

4 International Scientific Conference Agrobiodiversity Nutrition, Health and Quality of Human and Bees Life |129
th
September 11–13, 2019