Page 179 - Zbornik_Konf_2019

https://doi.org/10.15414/2019.9788055220703

4 International Scientific Conference Abstracts Book
th
CONTENT OF IRIDOIDS IN THE FRUITS OF LONICERA CAERULEA L.

Volodymyr Levon
M.M. Gryshko National Botanical Garden of the National Academy of Sciences of Ukraine
Kyiv, Ukraine; E-mail.: vflevon@gmail.com

To meet the needs of mankind in medical plant raw materials is constantly searching for
promising medicinal plants. Recently, much attention is paid to researchers’ non-traditional
berry crops, among which an important place is occupied by Lonicera caerulea L. The fruits of
L. caerulea exhibit anti-inflammatory, choleretic, diuretic and anti-ulcer effect, and the
presence of pectin increases the protective functions of the body when the whole body is
intoxicated with heavy metals. Eating fresh and frozen fruits are recommended for beriberi,
decreased appetite, General weakening of the body, atherosclerosis.
It is known that the medicinal properties of plants are due to the content of active
substances that have a certain effect on the human body. Iridoids belong to such compounds.
Unlike carotenoids or flavonoids, iridoids are quite resistant and do not break down during
prolonged storage or heat treatment of medicinal plants. Due to their bactericidal and
antioxidant properties, iridoids can be used as a natural preservative.
Pharmacological studies of iridoids isolated from different plants have shown a wide
range of their effects. The most valuable property of iridoids is its ability to be easily oxidized
and restored. Iridoids exhibit high biological activity-they provide antimicrobial, anti-
inflammatory, diuretic, antimicrobial and sedative action.
The purpose of our research is to determine the content of iridoids in the fruits of L.
caerulea for their use as medicinal raw materials. The object of research were varieties and
cultivars of L. caerulea: cultivars 'Skifska' and 'Golube vereteno', varieties No1 and No2 of the
collection of the M.M. Gryshko National Botanical Garden of NAS of Ukraine (NBG).
Iridoids content was determined by the method, which is the interaction of iridoid
compounds with hydroxylamine to form an oxime. The resulting oxime is capable of forming a
coordination compound with trivalent cations of iron, which has an absorption maximum at
512 nm. Control substance – garpagid. Extraction was performed with chloroform-ethanol
(5:1) and then, after removing the solvent by extraction with water. Changing the solvent
allowed to exclude the impact of related substances results in a hydroxamic reaction.
Comparative analysis of the content of iridoids in different cultivars and varieties of L.
caerulea from the collection of NBG showed that in fruits that do not have a bitter taste, there
are no iridoids. However, bitter forms and cultivars of L. caerulea accumulate iridoids. Thus, in
the bitter fruits of two varieties and cultivar of L. caerulea 'Skifska' their content was 0.08–
0.17 %. More iridoids have been found in fruits bitter-fruit cultivar 'Skifska'. The berries of
cultivar 'Golube vereteno', which sometimes have a bitter taste, was discovered iridoids, but in
a smaller quantity of 0.08 %. This indicates that in the more bitter fruits of L. caerulea the
content of iridoids is higher. In sprouts and leaves, L. caerulea iridoids are not found.
As a result of the studies, it was found that when using L. caerulea fruits as a source of
medicinal raw materials, it is desirable to use bitter-fruit forms that are characterized by a
higher content of iridoids.

Keywords: Lonicera caerulea, iridoids, fruits, comparative analysis.

4 International Scientific Conference Agrobiodiversity Nutrition, Health and Quality of Human and Bees Life |179
th
September 11–13, 2019