Page 183 - Zbornik_Konf_2019

https://doi.org/10.15414/2019.9788055220703

4 International Scientific Conference Abstracts Book
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VARIATION IN FUSARIOSE RESISTANCE GENES OF BETA VULGARIS L.
GENETIC RESOURCES
Arpine Nalbandyan, Tatyana Fedulova

A.L. Mazlumov All-Russian Research Institute of Sugar Beet and Sugar, Voronezh,
Russian Federation; E-mail.: arpnal@rambler.ru
Plant genetic resources are the major element of food safety of any country in the world.
It concerns Beta L. genus in full measure. Sugar beet is a valuable industrial crop cultivated to
produce the necessary foodstuff, namely, sugar. Its cultivation has started quite recently.
Cultivated beets are descendants from the Mediterranean species B. maritima. For stable use
of the crop introduced plants, it is necessary to conduct an estimation and selection for
presence of phytopathogen, Fusarium oxysporum Schltdl. in particular, resistance genes.
Aim of the investigations was to create specific DNA-markers, allowing identification of
fusariose resistance genes. Plants of 6 sugar beet varieties served as a material for the
investigations. Testing of plants for the presence of loci inherited linked to resistance
fusariosis genes was carried out using 3 specific primer pairs: EBS0085 (F/R), BB02714 (F/R)
and FusA (F/R).
To reveal the relation between SE2 and SP2 genes and sugar beet resistance to
fusariose root rot, experiments using the above-mentioned molecular markers were
performed. Amplification of plant DNA with the primer EBS0085 revealed the amplicon of 550
bp characteristic for the given marker in all samples. The homozygous (aa) 1, 5 and 6
genotypes (Р1537, Russia; Poli, Bulgaria; Kirghizskaya 069, Kirghizia) in electrophoretogram
(PCR amplification profile) had one distinct accurate and the most intensive amplicon
whereas the 2, 3 and 4 resistant heterozygotes (ab) were presented by PCR-fragments with
less expressed intensity and with an additional band of 700 bp. Amplification of plant DNA
with the primer BB02714 revealed the amplicon of 484 bp in the length characteristic for the
given marker in all samples. Amplification of plant DNA with the primer FusA also found out
the amplicon of 650 bp characteristic for the given marker in all genotypes. Thus, during the
investigations, sugar beet breeding samples presumably bearing genes of resistance to
fusariose rot in their genome were selected. All 6 investigated samples of sugar beet plants
(origin: Russia, Ukraine, Germany, Latvia, Bulgaria, Kirghizia) appeared to be carriers of R-
genes or loci inherited linked to genes of resistance to phytopathogens. These plants can be
recommended for use as a starting material in breeding for resistance to diseases. As used
here, however, the question is the presence of the required alleles. To determine the level of
their expression and phenotype display, real-time investigation of these genotypes using new
DNA-markers (SNP) and genetical sequencing is necessary.
Thus, the results of the conducted PCR-analyses indicate the need for further extending
of molecular-genetic investigations for selection of sugar beet forms with increased resistance
to diseases for optimization of a breeding process as a whole.
Keywords: sugar beet, resistance, Fusarium oxysporum, PCR-analysis, primers.

4 International Scientific Conference Agrobiodiversity Nutrition, Health and Quality of Human and Bees Life |183
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September 11–13, 2019