Page 170 - Zbornik_Konf_2019

https://doi.org/10.15414/2019.9788055220703

4 International Scientific Conference Abstracts Book
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VARIATIONS IN SEEDS MORPHOMETRICAL CHARACTERS OF DIOSPYROS LOTUS L.

Olga Grygorieva , Svitlana Klymenko , Yulia Vinogradova , Ján Brindza
1
1
2
3
1 M.M. Gryshko National Botanical Garden of the National Academy of Sciences of Ukraine,
Kyiv, Ukraine; E-mail.: olgrygorieva@gmail.com
2 N.V. Tsitsin Main Botanical Garden of Russian Academy of Sciences, Moscow, Russian Federation
3 Slovak Agricultural University in Nitra, Nitra, Slovak Republic
The study of seed morphology is an integral part of breeding work. Morphological
characters are used to determine the taxonomic status of samples, as well as to identify
duplication of germplasm collections, to assess genetic diversity, and for correlational study
between morphology and other important agronomic characters.
The aim of this study was to determine the variability of some morphometric characters
of seeds within an introduced population of Diospyros lotus. The results obtained will help us
to assess the diversity and stability in the genetic pool of the studied species formed in the
secondary cultigenous area.
The objects of the research were seeds plants of Diospyros lotus, which are growing into
Arboretum Mlynany (Slovakia) from the seeds acquired in 1970 from Korea, China and Japan.
There were 31 genotypes tested in an experimental study in 2018. Pomological
characteristics were made on a total of 100 seeds per genotypes. In the study only one plant
(tree) used for per genotype. Seeds were taken from ripe fruits in the autumn (November).
Fresh seeds were sterilized with distillate water and ethanol and cut along the groove.
Examined morphological parts were isolated and measured using a stereomacroscope Zeiss
Discovery V 12.
The morphometric parameters were following: seed length 10.41 (2.61–13.85) mm,
seed width 6.18 (1.35–8.25) mm, embryo length 5.85 (2.48–9.68) mm, radicle length 3.08
(1.05–6.14) mm, radicle diameter 0.81 (0.23–1.25) mm, cotyledon length 2.77 (1.42–4.42)
mm, cotyledon width 1.54 (0.50–2.54) mm. The shape indexes of seeds and cotyledon varied
01.84 (0.93–4.72) and 1.69 (0.39–2.37), respectively. The analysis of the coefficient of
variation showed the difference in variability of morphological signs between Diospyros lotus
samples. The most variability signs are the cotyledon width (21.40 %) and radicle diameter
(19.46 %). The results indicated very high correlations between the embryo length and the
embryo length (r = 0.953), embryo length and cotyledon length (r = 0.941). High correlations
observed between radicle length and cotyledon length (r = 0.801) and seed length (r = 0.768),
seed width and radicle diameter (r = 0.703).
Our results can be used to plan future research on the cultivation of Diospyros lotus L.
They will also include in the 'key' for identifying and describing new cultivars.

Keywords: Diospyros lotus L., seeds, morphometric parameters.

Acknowledgments
The publication was prepared with the active participation of researchers in international network
AgroBioNet, as a part of the international program 'Agricultural Biodiversity to Improve Nutrition,
Health and Quality of Life', within the project ITEBIO-ITMS 26220220115 'Support of Technologies
Innovation for Special Bio-Food Products for Human Healthy Nutrition' and National Scholarship
Programme of the Slovak Republic and Polish National Commission for UNESCO.

|170 4 International Scientific Conference Agrobiodiversity Nutrition, Health and Quality of Human and Bees Life
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September 11–13, 2019