Page 156 - Zbornik_Konf_2019

https://doi.org/10.15414/2019.9788055220703

4 International Scientific Conference Abstracts Book
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ANALYSIS OF PEANUT GERMPLASM VARIABILITY BY LENGTH POLYMORPHISM AMONG
THE PBS SITES OF RETROTRANSPOSONS
Jana Žiarovská , Eloy Fernández , Lucia Zamiešková , Julio Montero‐Torres , Sandra
3
1
1
2
Romero‐Ortega , Tania Pozzo , Jana Bezáková
4
1,5
3
1 Slovak University of Agriculture in Nitra, Faculty of Agrobiology and Food Resources, Department of
Genetics and Plant Breeding, Nitra, Slovak Republic;
E-mail.: jana.ziarovska@uniag.sk
2 Czech University of Life Sciences Prague, Faculty of Tropical AgriSciences, Department of Crop
Sciences and Agroforestry, Prague, Czech Republic
3 Major, Real and Pontifical University of San Francisco Xavier in Chuquisaca, Faculty of Agricultural
Sciences, Germplasm Bank – BIORENA, Sucre, Bolivia
4 University of California, Department of Plant Sciences,
Davis, California, United States of America
5 Slovak University of Agriculture in Nitra, Research Centre of AgroBioTech,
Nitra, Slovak Republic
Arachis hypogaea L. is a popular oil-seed crop that is a part of many national cuisines.
The diversity of uses of peanuts is an evidence of its antiquity – the seeds are eaten at one of
several stages from immature to fully ripe, raw, or cooked. They are processing by boiling,
broiling, roasting, crushing or grounding and mixing with other food. The aim of the study was
to analyse specific fingerprints of inter primer binding sites polymorphism of
retrotransposons in the genome of peanuts.
In total, twenty-one accessions were used in the study, collected in the in situ in Bolivia
as seeds and planted in pots. First, different iPBS markers were tested to be effective in
analysis and subsequently, three different markers were chosen. Amplification was
performed by following PCR program setup: initial denaturation 95 °C for 3 min, followed by
35 cycles of 95 °C for 30 s, 55 °C for 40 s, 72 °C for 2 min and a final extension at 72 °C for 5
min. Generated fingerprints profiles were evaluated for the presence or absence of individual
amplified loci. A Jaccard coefficient of genetic similarity was used together with UPGMA
analysis in PCoA.
Polymorphism level has ranged from 48 % up to the 75 % of used primer. Polymorphic
information content has ranged from 0.33 up to the 0.46 for the individual primers and
cophenetic coefficient has ranged from 0.83 up to the 0.91. None of the used iPBS markers
was considered to distinguish all of the analyzed accessions when evaluate obtained
fingerprints alone, but combining them in the final analysis, the level of genomic
polymorphism was sufficient to separate the analyzed Arachis hypogaea germplasm.
This study considers iPBS markers as sufficiently polymorphic in the genome of peanut
that will be useful in the assessment of its diversity and germplasm management for use in
breeding and conservation.
Keywords: peanut germplasm, DNA markers, variability, polymorphism.

Acknowledgement
This work was supported by AgroBioTech Research Centre built in accordance with the project
Building 'AgroBioTech' Research Centre ITMS 26220220180.

|156 4 International Scientific Conference Agrobiodiversity Nutrition, Health and Quality of Human and Bees Life
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September 11–13, 2019