Page 157 - Zbornik_Konf_2019

https://doi.org/10.15414/2019.9788055220703

4 International Scientific Conference Abstracts Book
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ANALYSIS OF SELECTED DROUGHT‐TOLERANCE GENES IN ALADIN AND SELADON
WHEAT CULTIVARS
Jana Žiarovská, Katarína Ražná, Veronika Štefúnová

Slovak University of Agriculture in Nitra, Faculty of Agrobiology and Food Resources, Department of
Genetics and Plant Breeding, Nitra, Slovak Republic;
E-mail.: jana.ziarovska@uniag.sk

Genes of drought resistance of common wheat (Triticum aestivum L.) DREB1 and DREB2
are located on the chromosome group 3 (A, B, D) of hexaploid wheat and they are present
usually in one copy. DREB3 is contiguous homologue to gene TmCBF5 and it was determined
on the chromosomes of groups 5, 6 and 7. The aim of the study was to characterize available
nucleotide records for mRNA and linear DNA of genes of drought resistance of common wheat
with using the bioinformatic algorithms and to compare their nucleotid record by sequencing
of the selected section for species Aladin and Seladon.
Sequencies of DREB2 gene are available in NCBI database in 26 records in total. By using
blastn algorithm was determined their similarity at the nucleotide conformity level. Direct
PCR amplicons sequencing was performed to obtain the partial sequence of DREB2 from two
analysed wheat varieties. Region of nucleotides 1707 – 2634 of T. aestivum_DREB2
gene_1BL_scaff_031713_54707-60515 (unpublished) was amplified and sequenced. PCR products
were purified by 1,5 IU FastAPTM alkalic phosphatase and 10 U of exonuclease I. Purified
amplicons were sequenced in direction 3´- 5´ by reverse amplification primer by GenomeLab™
DTCS. Nucleotide alignment algorithm was used to compare obtained sequences among
themselves and with those in public databases and a consensus sequence was generated.
The highest volume of similarity of nucleotide sequences of DREB2 genes was found
with the cover in rank 2–100 % and nucleotide conformity at volume 66–100 % for the
sequences stored in the public databases. Results of sequencing were the strength of 824
nucleotides for both of the analysed varieties. Any nucleotide differences between analysed
varieties were not determined by sequencing of the chosen section of DREB2 gene in Aladin
and Seladon even though they are differently physiologically based related to drought
resistance. When comparing the obtained sequences to the T. aestivum_DREB2
gene_1BL_scaff_031713_54707-60515 that was used for primer design, many nucleotide
insertions, substitutions and deletions were found.
Keywords: wheat; DREB2 gene, Aladin, Seladon.
Acknowledgement
This work was supported by project APVV-15-0156 'Genomická selekcia obilnín na suchovzdornosť'.

4 International Scientific Conference Agrobiodiversity Nutrition, Health and Quality of Human and Bees Life |157
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September 11–13, 2019