Page 123 - Zbornik_Konf_2019

https://doi.org/10.15414/2019.9788055220703

4 International Scientific Conference Abstracts Book
th
DEVELOPMENT OF TECHNOLOGY FOR CALLUS BIOMASS OF PLANTS OF
ASTERACEAE BERCHT. & J. PRESL FAMILY AND RANUNCULACEAE JUSS.
Romana Petrina, Sofia Suberlyak, Olha Shved, Viktoriia Havryliak,
Olena Fedorova, Zoriana Hubrii, Semen Khomyak

Lviv Polytechnic National University, Lviv, Ukraine; E-mail.: romanna.o.petrina@lpnu.ua
To preserve the natural reserves of plants, especially rare ones, on the verge of
extinction or to be included in the Red Book, and to provide quality, environmentally friendly
plant materials, a biotechnological method of growing in vitro is used. The method has many
advantages: biomass is obtained regardless of weather and season, in large quantities, does
not contain toxic substances and contains secondary metabolites present in the parent plant.
Medicinal plants are an important raw material base for traditional medicines, modern
medicines, nutrients, dietary supplements, traditional medicine, pharmaceutical intermediate
products, they have many biologically active compounds that can not be obtained by chemical
synthesis.
The purpose of this study was to obtain and research biomass of plants family
Asteraceae Bercht. & J. Presl and Ranunculaceae Juss. The study of seeds of Calendula
officinalis L., Arnica Montana L., Stevia rebaudiana (Bertoni) Bertoni, Carlina acaulis L., Adonis
vernalis L., Delphinium elatum L.
Methods. Seeds of plants were sprout in vitro on the Murasige-Skuha (MS) medium at 20
o С aseptically. Plants are transferred to the nutrient medium of MS with the addition of
growth regulators for the production of callus biomass. Individual conditions (temperature,
illumination, composition, and concentration of growth regulators) were selected for each
plant.
The callus biomass was dried, extracts were obtained which were investigated for the
presence of secondary metabolites. Extraction of plant material was also carried out. As an
extractant, 70 % ethanol is used. Extraction was carried out by infusion over 48 hours when
stirring. The extracts were filtered for further studies.
Qualitative and quantitative reactions have been made to determine phenolic
compounds, flavonoids, amino acids, vitamins A and C, alkaloids and terpenoids. The content
of secondary metabolites in plant callus biomass and plant biomass was comparable.
Results. As a result of the conducted studies, optimal compositions of nutrient media for
callusogenesis of plants family Asteraceae and Ranunculaceae were determined. Also, the
influence on the growth of callus biomass such parameters as the size of the explant, the
origin of the explant (root, stem, leaf), illumination was investigated. The best results are
obtained using explants of at least 0.4–0.5 mm, 2000 lux, 16/8 (light / dark) lighting. The time
of cultivation is offered – 40–50 days, depending on the plant. The technology of production of
callus biomass of plants is developed Calendula officinalis, Arnica montana, Stevia rebaudiana,
Carlina acaulis, Adonis vernalis, Delphinium elatum. The presence of secondary metabolites in
plant extracts and callus biomass has been confirmed, and a comparative analysis of the
results has been carried out.
Thus, the obtained results testify to the possibility of using callus biomass as an
alternative source of secondary metabolites of family Asteraceae and Ranunculaceae. The
developed technology has several advantages, namely profitability, ecological compatibility,
plant preservation, process automation.
Keywords: callus biomass of plants, Calendula officinalis, Arnica montana, Stevia rebaudiana, Carlina
acaulis, Caltha palustris, Adonis vernalis, Delphinium elatum.

4 International Scientific Conference Agrobiodiversity Nutrition, Health and Quality of Human and Bees Life |123
th
September 11–13, 2019