Page 118 - Zbornik_Konf_2019

https://doi.org/10.15414/2019.9788055220703

4 International Scientific Conference Abstracts Book
th
STUDY OF MINERAL COMPOSITION IN THE LEAVES OF AMARANTUS L.

Svetlana Motyleva , Murat Gins , Valentina Gins , Ivan Kulikov , Ekaterina Gins ,
2
1
2
2
1
Viktor Pivovarov , Sergei Medvedev 1
2
1 Federal State Budgetary Scientific Institution All-Russian Horticultural Institute for Breeding,
Agrotechnology and Nursery, Moscow, Russian Federation; E-mail.: motyleva_svetlana@mail.ru
2 Federal Center of Vegetable Production, Moscow, Russian Federation
In photosynthetic organisms, the accumulation of calcium oxalate crystals at high levels
is a normal activity tightly integrated with metabolism. The huge variation in distribution
among organs, tissues, and cells among plant species suggests multiple independent origins of
crystal formation and its functions. Despite their ubiquity, knowledge on the formation and
the possible role of these crystals remains limited. Calcium oxalate crystals are a major
biomineralization product in higher plants. Their biological function and use are not well
understood. In this work, we focus on the isolation and crystallochemical characterization of
calcium oxalate crystals in the leaves of Amarantus – is an annual plant.
The objects of research are the leaves Amaranthus tricolor L. breeds by Federal center of
vegetable production selection (Moscow region, Odintsovo). The plants were grown in the
open on the experimental fields, leaves samples for analysis were collected. The
determination data of quantitative elemental composition, presented in this paper, are
fulfilled in the laboratory of electronic microscopy of FSBSI ARHIBAN Plants genofund and
bioresources center, Moscow.
Тhe anatomical features end chemical composition of the inclusions was studied by EDS,
combined with an analytical scanning electron microscope JEOL JSM 6090 LA.
On the transverse sections of the amaranth leaves and in the veins of the leaf, multiple crystals
of circular shape were found.
Symmetric protrusions of crystals are viewed on the SEM image of the abaxial and
adaxial sides of the leaves Amarantus L.
EDS analysis revealed 4 elements in the crystal – Mg (0.25), P (0.48), K (0.16) and Ca
(38.59) mass%.
The distribution of Mg, P, K, Ca in the mineral inclusions. All elements were clearly
observed and mapped using Smiling program. X-ray images which mapped all elements
location K, P and Mg show that are distributed uniformly around of the mineral inclusions. Ca
is concentrated in the crystal. The SEM / EDX results are allowed to determine the
concentration and distribution of elements in the mineral inclusions in the leaves Amarantus.
The crystal cells or idioblasts display ultrastructural modifications which are related to
crystal precipitation.
The combination of SEM and EDX was a convenient method for determining the mineral
inclusions in the cross-section leaves Amarantus. It was found that Ca is localized in the
granule. Probably the crystals perform a supporting function in the sheet structure.
Keywords: Amaranthus tricolor, leaves, analytical scanning electron microscopy.

|118 4 International Scientific Conference Agrobiodiversity Nutrition, Health and Quality of Human and Bees Life
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September 11–13, 2019