Page 85 - Zbornik_Konf_2019

https://doi.org/10.15414/2019.9788055220703

4 International Scientific Conference Abstracts Book
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BIOLOGICAL ACTIVITY OF LEAVES OF NON‐TRADITIONAL PLANTS

Olga Grygorieva , Svitlana Klymenko , Olena Vergun , Narcyz Piórecki , Ján Brindza ,
1
1
1
2
3
Eva Ivanišová 3
1 M.M. Gryshko National Botanical Garden of the National Academy of Sciences of Ukraine,
Kyiv, Ukraine; E-mail.: olgrygorieva@gmail.com
2 Arboretum and Institute of Physiography in Bolestraszyce, Przemysl, Poland
3 Slovak University of Agriculture in Nitra, Slovak Republic
Fruits constitute is a large group of functional food, whose consumption delivers several
health benefits, but very limited studies about the leaves of plants especially lesser-known
and non-traditional plants, among which fruit plants. Leaves of non-traditional plants are one
of the promising sources of antioxidants. They can use in the tea production and may have
potential health benefits as a therapeutic aid in many illnesses which could be attributed to
their antifungal, anti-inflammatory, antimicrobial and antioxidant activities.
The aim of the research is the determination of the total antioxidant activity and the
content of phenolic compounds of the leaves of 10 species of non-traditional plants, namely,
Amelanchier alnifolia (Nutt.) Nutt. ex M. Roem., Aronia mitschurinii A.K. Skvortsov & Maitul.,
Castanea sativa Mill., Cornus mas L., Diospyros kaki L., Diospyros lotus L., Diospyros virginiana
L., Chaenomeles japonica (Thunb.) Lindl., Pseudocydonia sinensis (Thouin) C.K. Schneid.,
Ziziphus jujuba Mill.
The raw materials were collected in the 2018 July on the experimental collection of
Institute of Biological Conservation and Biosafety of Slovak University of Agriculture in Nitra.
Biochemical analyses conducted in the laboratory of the Department of Technology and
Quality of Plant Products. Total phenolic content (TPC) was evaluated using the Folin-
Ciocalteu reagent assay. Antioxidant activity (AOA) was measured using two different
methods (DPPH – 2,2-diphenyl-1-picrylhydrazyl, MRAP – molybdenum reducing antioxidant
power).
Significant variability was observed in phenolic compounds content and total
antioxidant activity. Total polyphenol content ranged from 38.02 (Z. jujuba) to 80.58
(C. sativa) mg GAE/g DW, total flavonoid content from 22.47 (P. sinensis) to 54.35 (Z. jujuba)
mg QE/g DW and phenolic acids content from 3.51 (A. mitschurinii) to 24.67 (Ch. japonica) mg
CAE/g DW. All tested samples exhibited DPPH• radical scavenging activities with values from
6.92 (A. mitschurinii) to 9.0 (C. mas) mg TEAC/g DW. Antioxidant activity by molybdenum
reducing antioxidant power method ranged from 109.43 (A. mitschurinii) to 322.95 (C. mas)
mg TEAC/g DW. Differences between the species of non-traditional plant were significant in
all observed parameters.
The plants leave extracts consistently exhibited the highest antioxidant activity.
Obtained results of phytochemical composition demonstrated the possibility of leaves use of
non-traditional plants as sources of valuable bioactive compounds with health-promoting and
disease-preventing properties. However, are recommended further studies considering the
complex of factors (conditions of growth, stage, etc.) that influence the accumulation of
biologically active compounds.

Keywords: non-traditional plants, leaves, antioxidant activity.
Acknowledgments
The publication was prepared with the active participation of researchers in international network
AgroBioNet, as a part of the international program 'Agricultural Biodiversity to Improve Nutrition,
Health and Quality of Life', within the project ITEBIO-ITMS 26220220115 'Support of Technologies
Innovation for Special Bio-Food Products for Human Healthy Nutrition' and National Scholarship
Programme of the Slovak Republic and Polish National Commission for UNESCO.

4 International Scientific Conference Agrobiodiversity Nutrition, Health and Quality of Human and Bees Life |85
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September 11–13, 2019