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https://doi.org/10.15414/2019.9788055220703
4 International Scientific Conference Abstracts Book
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SCANNING ELECTRON MICROSCOPY STUDY OF POLLEN MORPHOLOGICAL CHARACTERS
OF SAMBUCUS NIGRA L.
Vladimíra Horčinová Sedláčková , Ivan Gurnenko , Ján Brindza
1
2
1
1 Institute of Biodiversity Conservation and Biosafety, Slovak University of Agriculture in Nitra, Slovak
Republic; E-mail.: vladimira.sedlackova@uniag.sk
2 M.M. Gryshko National Botanical Garden of the NAS of Ukraine, Kyiv, Ukraine
The pollen grains have a definite shape, size, color, structure for each species, genus and
family and these characters are useful for systematic botany. The complex of these
morphological characteristics and ultrastructure allows determining the differences or
similarities between the same and various species and genotypes.
In the Slovak flora, Sambucus nigra L. is the most vegetative species from the Adoxaceae
E.Mey. family, but pollen grains were not yet studied using by SEM (Scanning Electron
Microscopy) in the conditions of Slovakia.
The aim of the work was to study the general characteristics and significant
morphological traits of Sambucus nigra pollen grains. The studies were performed on pollen
obtained from wild-growing twenty Sambucus nigra varieties (SN-1–SN-18) and cultivar
Haschberg (SN-19–SN-20) cultivated in Slovakia. The pollen grains were studied at the
Institute of Biodiversity Conservation and Biosafety in Nitra (Slovakia) and the Laboratory of
Department of Tropical and Subtropical plants of M.M. Gryshko National Botanical Garden
(Kyiv, Ukraine) using the scanning and transmission electron microscopy SEM. The
measurement of morphometric parameters was carried out on 50 pollen grains from each
genotype using the AxioVision Rel. 4.8.2.0 program.
The shape of fresh pollen grains is slightly elongated, closer to spheroidal. In outline
from the pole – round. The form of dried pollen grains is oval. Sambucus pollen is three-
furrowed. Furrows are long, with oval, even edges. Exina sculpture is coarse-mesh.
This study showed that there were significant differences the samples in all measured
factors. The polar axis of pollen grains values varied from 18.23 to 29.91 μm (varieties) and
from 20.99 to 26.85 μm (cv. Haschberg). The equatorial diameter values for wild-growing
varieties and cv. Haschberg pollen grains varied from 10.86 to 16.83 μm and from 12.28 to
16.43 μm, respectively. There were no significant differences between pollen grains of wild-
growing varieties and cultivar Haschberg. This study confirmed small differences among the
genotypes in all measured factors with variation coefficient in the range 2.62–8.62 %. Shape
index of tested genotypes varied from 1.08 to 2.36 for wild-growing varieties and from 1.30 to
2.08 for cv. Haschberg.
It was noted that diversity of surface sculpturing of pollen grains in combination with
shape and sizes of them enables to use complex of thin morphologic signs for Sambucus nigra
pollen identifications.
Keywords: Sambucus nigra, pollen, SEM, morphology, Slovakia.
Acknowledgments
The publication was prepared with the active participation of researchers in international network
AgroBioNet, within the project ITEBIO-ITMS 26220220115. Co-author (51810109) is grateful to
Visegrad Scholarship Fund for the scholarship grant and National Scholarship Programme of the
Slovak Republic for the research stay during which the presented knowledge was obtained.
|86 4 International Scientific Conference Agrobiodiversity Nutrition, Health and Quality of Human and Bees Life
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September 11–13, 2019