Page 90 - Zbornik_Konf_2019

https://doi.org/10.15414/2019.9788055220703

4 International Scientific Conference Abstracts Book
th
EXPERIENCE OF OBTAINING OF PLANT TISSUE CULTURES OF CACTACEAE JUSS.

Roman Ivannikov , Oksana Lobova , Natalia Ivannikova ,
1
1
2
Iryna Krasnenkova 1
ˡM.M. Gryshko National Botanical Garden, Kyiv, Ukraine; E-mail.: ivannikov@nbg.kiev.ua
2 National University of Life and Environmental Sciences of Ukraine, Kyiv, Ukraine
The state Botanical Institutes of Ukraine keep unique, ones of the biggest collections of
succulent plants in Eastern Europe. Among them, Cactaceae Juss. is one of the numerous
families of Angiosperms which inhabit arid parts of New World. Many of them are important
agricultural species. In fact, most of the species of Cactaceae are in danger of extinction or
they exist only in collections.
According to the mentioned above, as objects of study, three rare species of cacti were
selected that are endangered in natural habitats.
Astrophytum asterias (Zucc.) Lem. is in the most endangered position. The species
belong to Appendix I of the СІТЕС and to the vulnerable species category of IUCN. Blossfeldia
liliputiana Werderm. is a monotypic genus of cacti that belong to Appendix II of CITES.
Strombocactus disciformis (DC.) Britton & Rose is a monotypic species of the genus, endemic
species of Mexico which belong to the red list of IUCN (status “vulnerable species”) of
Appendix I of СІТЕС and to the National List of endangered plant species of Mexico.
The aim of our study is to obtain sterile tissue cultures, to investigate the particularities
of organogenesis on the initial stages of ontogenesis and approbation of micropropagation
procedure of the mentioned taxonomic groups of Cactaceae.
The results of the study are represented by the approbated procedure of sterilization of
intact material and obtained sterile cultures of these species (ethanol (70 %) – 0.5 min;
sterilized distilled water (SD) – 5 min; thimerosal (0.1 %) – 16 min; SD – 10 min; Domestos –
8 min; Н2О2 (10 %) – 10 min; SD – 10 min). The expediency of the use as primary explants
stems of juvenile plants grown in vitro from embryos is shown.
The possibility of obtaining of the necessary amount of plant material by the way of
indirect organogenesis is proved. The conditions and content of nutrient media for obtaining
their primary callus are selected.
It was defined that for the experimental species of Cactaceae, light and space orientation
of the explant are the key factors in the primary morphogenic callus formation. The conditions
of the successful induction of the formation of the morphogenic callus and initiation of
hemorhyzogenesis in the sterile conditions are defined. The contents of nutrient media and
conditions of cultivations for all stages of micropropagation were selected. The regenerated
plants of experimental species of cacti were obtained, and the procedure of the conditions of
their postaseptic adaptation was approbated.
Keywords: in vitro, Astrophytum asterias, Blossfeldia liliputiana, Strombocactus disciformis.

Acknowledgments
The experiments of the study were accomplished on the basis of the sterile tissue cultures collection of
the Tropic and subtopic plants apartment of the M.M. Gryshko National Botanical Garden that from
1999 has a status of National Heritage of Ukraine and is supported by the definite state program.

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September 11–13, 2019