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https://doi.org/10.15414/2019.9788055220703

            4  International Scientific Conference                                           Abstracts Book
              th
                      DIVERSITY OF POLLEN GRAINS OF TILIA GENUS IN UKRAINIAN HONEYS

                             Leonora Adamchuk , Oleksandr Shynder , Jana Šimková
                                                                                           3
                                                                          2
                                                   1
                             1 National University of Life and Environmental Sciences of Ukraine,
                                   Kyiv, Ukraine; E-mail.: leonora.adamchuk@gmsil.com
                   2 M.M. Gryshko National Botanical Garden of the National Academy of Sciences of Ukraine,
                                                      Kyiv, Ukraine
                  3 Institute of Biological Conservation and Biosafety, Slovak University of Agriculture in Nitra,
                                                  Nitra, Slovak Republic
                  One of the most valuable varieties of Ukrainian honey is linden, known for its medicinal
            properties and organoleptic commodity qualities (taste, aroma). However, exporters often
            face the problem of identifying pollen grains of certain species of Tilia spp. in honey, which
            makes it impossible to position them as a varietal. That is why studying the morphological
            features of pollen grains of the species of lindens growing on the territory of our state for the
            purpose of further their recognition in honey has a significant scientific and practical
            significance. Features of the structure and surface of pollen grains of the  Tilia genus were
            studied by various scientists using scanning  electronic microscopy (Chambers & Godwin,
            1961; 1971; Andrew, 1971; Stockmarr, 1974). However, profound scientific research, in the
            vast majority, concerned two types – T. cordata Mill. and T. platyphyllos Scop. In the modern
            database of pollen grains for identification of them there are only four species of lindens – T×
            euchlora K. Koch,  T. cordata,  T. platyphyllos  and  T. tomentosa Moench (www.paldat.org); and
            only two of them  (T. euchlora  and  T. tomentosa)  have an image of a pollen under a light
            microscope, which is suitable for identification of the species in honey. At the same time, there
            are other resources for identification of linden species. For example, Science and Plants for
            Schools (SAPS) (www.saps.org.uk), which offers images of three species of lindens –
            T. platyphyllus, T. × euchlora, and T. × europea. The Pollen-Wiki resource also offers images of
            only three species of linden –  T. Henryana Szyszyl.,  T. platyphyllos, and  T. cordata.  Atlas of
            pollen grains of russian authors, which is widely used to identify the botanical origin of honey
            from Eastern Europe and Asia, has identifiers for only two species of lindens – T. begoniifolia
            Steven and T. cordata.
                  In the flora of Ukraine, there are 5 native species of the Tilia genus and 2 hybridogenic
            species. In addition, in plantations and parks  throughout the territory of Ukraine, it is
            cultivated more than 10 introduced species of the genus. This causes a sharp need to study
            the morphology of their pollen  grains for further identification of honey. For a separate
            direction of experimental work, we have selected to study the morphological features of
            pollen grains of various forms  of the hybridogenic species  T.  ×  europea (T. cordata  ×
            T. platyphyllos), which is one of the earliest flowering species, is widely distributed in parks
            and other artificial plantations and has high seed reproduction rates.
            Keywords: Tilia, pollen grain, Ukrainian honey, linden honey.

            Acknowledgments
            Author Leonora Adamchuk thanks the International Visegrad Fund (51910842) for the scholarship
            and research internships, during which the results and knowledge presented in this paper were
            obtained. The publication was prepared with the active participation of researchers involved in the
            International network AgroBioNet of the Institutions and researchers for the realization of research,
            education, and development program 'Agrobiodiversity  for Improving  Nutrition, Health and Life
            Quality' TRIVE (ITMS 26110230085).










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