Page 116 - Zbornik_Konf_2019

https://doi.org/10.15414/2019.9788055220703

4 International Scientific Conference Abstracts Book
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ANTIOXIDANT ACTIVITY OF AROMATIC HERBS FROM BOTANICAL GARDEN OF THE
SLOVAK UNIVERSITY OF AGRICULTURE IN NITRA
Erika Mňahončáková , Olena Vergun , Olga Grygorieva ,
2
2
1
Vladimira Horčinová Sedláčková , Jana Šimková , Ján Brindza , Eva Ivanišová 4
3
3
3
1 Botanical Garden of Slovak University of Agriculture in Nitra, Slovak Republic;
E-mail: erika.mnahoncakova@gmail.com
2 M.M. Gryshko National Botanical Garden of the NAS of Ukraine, Kyiv, Ukraine
3 Institute of Biological Conservation and Biosafety, Slovak University of Agriculture in Nitra,
Slovak Republic
4 Department of Technology and Quality of Plant Products, Slovak University of Agriculture in Nitra,
Slovak Republic
Biological activity of plant raw is an actual topic in modern biological science. Study of
antioxidant activity of plants the most discussed direction in scientific references.
Plant material of this study was Hyssopus officinalis L., Lavandula angustifolia Mill.,
Nepeta kokanica (Regel) Kuntze, Rosmarinus officinalis L., Salvia officinalis L., Thymus
serpyllum L., and Th. vulgaris L. harvested from the collection of Botanical Garden of Slovak
University in Nitra at the period of flowering. It was determined total content of polyphenols
(gallic acid equivalent (GAE)), flavonoids (quercetin equivalent (QE)), phenolic acids (caffeic
acid equivalent (CAE)), antioxidant activity (Trolox equivalent (TE)) and reducing power (TE)
of extracts. All measurements carried out on the spectrophotometer Jenway (6405 UV/Vis,
England).
In our study total content of polyphenols among investigated plant extracts was from
39.43 to 62.87 mg GAE/g of DW (dry weight) and increased in the following order: L.
angustifolia > Th. serpyllum > H. officinalis > R. officinalis > N. kokanica > Th. vulgaris > S.
officinalis. The content of flavonoids was from 20.80 to 36.80 mg QE/g of DW and increased in
the following order: Th. serpyllum > L. angustifolia > H. officinalis > Th. vulgaris > N. kokanica >
R. officinalis > S. officinalis. Content of phenolic acids of alcohol extracts was from 4.91 to
24.30 mg CAE/g of DW and increased in next order: N. kokanica > R. officinalis > L. angustifolia
> H. officinalis > Th. serpyllum > Th. vulgaris > S. officinalis. Antioxidant activity by DPPH-
method was from 7.78 to 8.50 mg TE/g of DW and increased in the following order: S.
officinalis > L. angustifolia > R. officinalis > Th. serpyllum > N. kokanica > Th. vulgaris > H.
officinalis. Reducing power of investigated extracts was from 122.92 to 225.36 mg TE/g of DW
and increased in the following order: N. kokanica > Th. serpyllum > Th. vulgaris > L.
angustifolia > S. officinalis > H. officinalis.
Thus, we found that among investigated plants S. officinalis extracts had the highest
amount of polyphenol compounds, phenolic acids, and flavonoids. Antioxidant activity and
reducing power was maximal for plants of H. officinalis. All investigated plants are a potential
source of natural antioxidants and have potential as therapeutic agents.

Keywords: aromatic herbs, antioxidant activity, polyphenols.
Acknowledgments
This study was financially supported by scholarships from International Visegrad Fund (51810415),
National Scholarship Programme of the Slovak Republic and Polish National Commission for UNESCO.
This study also conducted within the framework of AgroBioNet.

|116 4 International Scientific Conference Agrobiodiversity Nutrition, Health and Quality of Human and Bees Life
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September 11–13, 2019