Page 191 - Zbornik_Konf_2019

https://doi.org/10.15414/2019.9788055220703

4 International Scientific Conference Abstracts Book
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ESSENTIAL OILS OF LAVANDINS AND THEIR ANTI‐CANDIDA ACTIVITY

Natalia Vorobets , Halina Yavorska , Ludmyla Svydenko , Zinovii Vorobets 1
1
2
3
1 Danylo Halytsky Lviv National Medical University, Lviv, Ukraine;
E-mail: vorobetsnatalia@gmail.com
2 Ivan Franko National University of Lviv, Ukraine

3 Institute of Rice of National Academy of Agrarian Sciences of Ukraine, Plodove, Ukraine
Candida spp. are the most common etiological agents of candidiasis, causing not only superficial
mucosal candidiasis but also life-threatening systemic infection especially in immunocompromised
patients. The presence of clinical strains of Candida, resistant to currently used chemically synthesized
antifungal agents, causes the urgent need for the development of new antifungal drugs based on
substances of natural origin. Lavandins Inii and Rabat are interspecies hybrids obtained as the results
of natural and artificial selection of Lavandula angustifolia Mill. and Lavandula latifolia Medic.
(Svydenko, 2001). Phytochemical screening of Inii and Rabat active components revealed a high
content of essential oils (EOs).

The aim of this study is to show the anticandidal activity of Inii and Rabat lavandins EOs against
the clinically important yeasts: Candida pseudotropicalis, C. parapsilosis, C. curvata, C. kefir, and C.
tenuis. EOs of collected lavandins’ Inii and Rabat inflorescences were extracted by hydrodistillation in
a Clevenger apparatus. The strains used from the Microbial Culture Collection of the Department of
Microbiology of Ivan Franko National University of Lviv. The standard agar well diffusion method was
used to determine the sensitivity of microorganisms to EOs. The samples of lavandins’ Inii and Rabat
EOs in the amount of 100 µL or 200 µL were applied. Decasanum and Essential Oil of Lavender
(Lavandula angustifolia Mill. of the genus Lavandula (family Lamiaceae) were used as controls.

Results indicate that all Candida strains used during the present study are completely sensitive
to essential oils of lavandins Inii and Rabat. Both the tested oils demonstrated inhibitory action against
all the Candida species at low concentration. The most sensitive were C. pseudotropicalis, C.
parapsilosis, and C. kefir, some less sensitive were C. curvata and C. tenuis. The anticandidal activity of
the essential oils of both investigated lavandins may be related to their major volatile compounds,
namely linalool, linalyl acetate, 1.8-cineole, and camphor. It is more likely that the anticandidal effect is
provided by the joint action of all the components present in the composition of the essential oils. It
was found that the anti-candidal activity of EOs of lavandins Inii and Rabat is comparable to that of
EOs of lavender (Lavandula angustifolia) and significantly exceeds the efficiency of Decasanum.
Essential oils of lavandins Inii and Rabat are a promising use in healthcare decontamination
against Candida spp. The ability to obtain a sufficient amount of plant material of lavandins in Kherson
region indicates a good prospect of further research and the use of their essential oils on an industrial
scale.
Keywords: lavandin, essential oil, anti-candida.

4 International Scientific Conference Agrobiodiversity Nutrition, Health and Quality of Human and Bees Life |191
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September 11–13, 2019