Page 189 - Zbornik_Konf_2019

https://doi.org/10.15414/2019.9788055220703

4 International Scientific Conference Abstracts Book
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AMINO ACID COMPOSITION OF LEAVES, FRUITS, SEEDS AND CALYX OF
AMERICAN PERSIMMON (DIOSPYROS VIRGINIANA L.)
Yulia Zhabrovets , Yevhen Kustovskiy , Alla Kustovska , Olga Grygorieva ,
2
1
1
1
Zuzana Schubertová
3
1 National Pedagogical Dragomanov University, Kyiv, Ukraine; E-mail.: additive@ukr.net
2 M.M. Gryshko National Botanical Garden of the National Academy of Sciences of Ukraine,
Kyiv, Ukraine
3 Institute of Biological Conservation and Biosafety, Slovak University of Agriculture in Nitra, Slovak
Republic
Diospyros virginiana L. (American persimmon) since ancient times are used in folk
medicine. The fruit has been used medicinally as antiseptic and for the treatment of burns,
diphtheria, dropsy, diarrhea, gonorrhea, candidiasis, dysentery, fevers, thrush, fungal and
bacterial infections, gastrointestinal bleeding, sore throats, exhibit antimicrobial and
antifungal activities. Also, the persimmon fruit can be considered as a highly nutritional
product because of its strong antioxidant capacity induced by a high content of flavonoids,
vitamin C, beta-carotene. Biologically active compounds are located not only in fruits but in
different parts of the plant: bark, wood, leaves, roots. The bark has antiseptic properties,
hepatoprotective and antipyretic activity, the leaves showed antimycobacterial effect, the
roots of American persimmon showed antifungal effect. Powder of dry leaves has been used
for a long time in folk medicine. The leaves of Diospyros virginiana found lupeol, betulin,
betulinic acids – components that are famous with their antitumor properties.
The aim of this study was to investigate qualitative and quantitive content of amino
acids compounds of leaves, fruits, seeds and calyx of Diospyros virginiana from which are
growing in Forest-Steppe of Ukraine in M.M. Gryshko National Botanical Garden of NAS of
Ukraine (NBG). The content and composition of amino acids determined on automatic amino
acid analyzer AAA 339 M (MIKROTECHNA, Chech Republic).
As a result of the research, it has been found that the fruits amino acids (alanine,
asparagine, cysteine, glycine, glutamine, proline, serine, tyrosine) in an amount from 0.1 to
1.4 g/kg . Seeds contained from 0.6 to 5.6 g/kg , leaves from 2.2 to 17.4 g/kg and calyx
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from 2.9 to 12.6 g/kg . Also, the content of essential amino acids arginine, histidine,
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isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, valine was
distributed as it follows: in fruits from 0.2 to 2.1 g/kg , in seeds from 0.01 to 3.5 g/kg , in
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leaves from 2.3 to 12.3 g/kg , in calyx from 1.3 to 23.5 g/kg . The result of the research
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allows us to state that the highest level of amino acids can be found in leaves and calyxes.
Among the essential amino acids (g/kg ), the highest content in leaves was 12.3 (arginine),
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10.1 (leucine), 8.1 (valine), among non-essential amino acids 17.4 (glutamine),
10 (asparagine), 6.8 (alanine). In the calyxes, the highest content was 23.5 (histidine),
12.6 (glutamine), 11.3 (asparagine), 9.6 (leucine), 8.4 (valine), 7.3 (alanine).
Obtained results of this study indicated about high and diversified content of amino
acids and allow to suggest the availability of wide specter of pharmacological activity of
leaves, fruits, seeds, and calyx Diospyros virginiana. This indicates on the perspective of the
use of investigated raw (particularly, leaves и calyx) as a source of essential and non-essential
amino acids, and also can be used for in-depth study of Diospyros virginiana as a source of
other biologically active compounds.

Keywords: Diospyros virginiana, amino acids, leaves, fruits, seeds, calyx.
Acknowledgments
The publication was prepared with the active participation of researchers in international network
AgroBioNet, within the project ITEBIO-ITMS 26220220115 'Support of Technologies Innovation for
Special Bio-Food Products for Human Healthy Nutrition' and National Scholarship Programme of the
Slovak Republic and Polish National Commission for UNESCO.

4 International Scientific Conference Agrobiodiversity Nutrition, Health and Quality of Human and Bees Life |189
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September 11–13, 2019