Page 163 - Zbornik_Konf_2019

https://doi.org/10.15414/2019.9788055220703

4 International Scientific Conference Abstracts Book
th
SCORZONERA HISPANICA L. – PROMISING BIOTECH CULTURE

Olga Bulko, Lyudmila Lioshyna
Institute of Cell Biology and Genetic Engineering National Academy of Sciences of Ukraine, Kyiv,
Ukraine; E-mail.: obulko@ukr.net
In recent decades, diseases associated with unhealthy diets, such as obesity, diabetes,
atherosclerosis, and others have become widespread among the world's population. In this
regard, the issues of revising the usual diet and introducing new or undeservedly forgotten
healthy food products are of particular relevance. Black salsify Scorzonera hispanica L. from the
family Asteraceae Bercht. & J. Pres is just such an undervalued plant because its edible root
crops contain a significant amount of dietary fiber, which has an insignificant energy value, but
regulate the physiological and biochemical processes in the digestive organs, removing harmful
substances from the human body. Scorzoner contains soluble fructan –inulin (up to 38 % of the
dry weight), which is characterized by a low content of glucose in the molecule. This makes it a
particularly valuable alternative source of carbohydrates for diabetics since insulin is not
required to digest inulin. In addition, in this plant there is a lot of protein, micro- and macro-
elements, vitamins, other bioactive substances, radioprotective and anti-cancer properties are
also found. The reproduction of black salsify by seeds is difficult since the seeds lose their
germination in the second year, therefore the microclonal propagation of this dietary plant is
relevant.
For the introduction of S.hispanica seeds into in vitro culture, treatment with potassium
permanganate, 70 alcohol and 0.1 % HgCl2 solution was used. Germination was low, about 23
O
%. The culture medium for reproduction on the basis of Murasige-Skoog (MS) was supplemented
with various combinations of plant growth regulators: 6 benzylaminopurine (6-BAP), kinetin (6-
furfuryl-aminopurine), indolyl-3-acetic acid (IAA), indolyl butyric acid (IBA), α-naphthylacetic
acid (NAA) in a concentration of from 0.1 to 1.0 mg/l. The maximum multiplication factor was
observed when plant tissue culture medium was supplemented with 0.5 mg/l 6-BAP (up to
12.3), however, the resulting plants were very small and unsuitable for further passage. In
addition, the plant proved to be demanding on the source of iron in the medium, the standard
amount of ferrum chelate was insufficient for normal development of scorzoner, and the use of
the so-called red iron (ethylenediamine di-2-hydroxyphenyl acetate ferric (Fe-EDDHA)) was also
not effective. It was possible to achieve an intensely green, non-chlorotic coloration of plants,
only by increasing the content of ferrum chelate by a factor of 2 compared with the standard
recipe. The optimal medium for micropropagation of S.hispanica was the medium with the
addition of 0.5 mg/l of kinetin and 0.01 mg/l of NAA when the coefficient decreased to 7.8, but
the obtained microclones were larger, morphologically homogeneous, with a high survival rate
when the plants were passaged. Rooting did not occur on the hormone-free medium MS,
different concentrations of auxins were tested to stimulate root formation, the maximum
number of roots was observed on the medium with the addition of 0.7–0.8 mg/l IBA.
Thus, the conditions were selected for the micropropagation of the Scorzonera hispanica –
a promising food plant and a source of inulin.
Keywords: Scorzonera hispanica L., micropropagation, phytohormones, inulin.

4 International Scientific Conference Agrobiodiversity Nutrition, Health and Quality of Human and Bees Life |163
th
September 11–13, 2019